PepperSS-0092Heat LadderPepperProof of Concept

Venom Protocol

C. chinense · Heat Ladder: Venom Protocol

Scoville Heat Units

2,052,288

16M

12.8% of pure capsaicin

This is a computed design target (12.8% of pure-capsaicin concentration), derived from the flux model and blend chemistry — not a measured value. It is above anything measured in a pepper to date. See the evidence behind it:

Paper & methods Datasheet Validation status How we know

Genes

Construct

14,655 bp

Variant

Triple

Promoter

2A11

Technical Manifest

Construct Type

T-DNA binary vector

Selection Marker

nptII (kanamycin resistance)

Codon Optimization

pepper

Capsaicinoid Profile

extreme multi

Gene Cassettes (9)

pAMT expression cassettevanillylamine biosynthesis

Pun1/CS (Triple) expression cassettecapsaicin synthase

BCAT expression cassettepathway enzyme

KAS expression cassettepathway enzyme

FAR expression cassettepathway enzyme

PAL expression cassettepathway enzyme

C4H expression cassettepathway enzyme

4CL expression cassettepathway enzyme

HCT expression cassettepathway enzyme

Construct map

14,655 bp · T-DNA binary vector

Left Border

nptII selection

pAMT expression

Pun1/CS expression

BCAT expression

KAS expression

FAR expression

PAL expression

C4H expression

4CL expression

HCT expression

Right Border

Left Border (LB) · 78 bp

nptII selection cassette · 795 bp

pAMT expression cassette

Pun1/CS (Triple) expression cassette

BCAT expression cassette

KAS expression cassette

FAR expression cassette

PAL expression cassette

C4H expression cassette

4CL expression cassette

HCT expression cassette

Right Border (RB) · 75 bp

Burn Profile

Heat Ladder: Venom Protocol

Capsaicinoid blend: extreme multi

Onset 1s

Peak 5s

Duration 2m

Profile Guillotine

Specimen library

Organism documentation

On-plant

Cross-section

Clinical specimen

Download

This is one of our 10 founding pepper constructs — the proof of concept for the entire Scoville Splice platform. The full package is yours: academic paper, FASTA construct, datasheet, manifest, FBA predictions, CRISPR guides, and quick-start protocol.

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Read Full Paper →View Datasheet →Analyze in Platform →

Common questions

What is the target SHU of Venom Protocol?

Venom Protocol is engineered to a target of 2,052,288 Scoville Heat Units. Burn character: Heat Ladder: Venom Protocol. Capsaicinoid profile: extreme multi.

What host organism does Venom Protocol use?

Venom Protocol is a Pepper cultivar in the Heat Ladder series, built on Pepper (C. chinense). The construct runs 9 genes across 14,655 base pairs, driven by the 2A11 promoter system.

What license tiers are available for Venom Protocol?

Every catalog cultivar can be licensed at Specimen (one SKU, non-exclusive) or Sector (industry-sector exclusive) tiers. Genome and Platform licensing are available on request for category or stack-wide deals. See the licensing page for pricing structure.

What is included in the Venom Protocol download package?

The Venom Protocol bundle contains the full construct FASTA, a JSON manifest (gene list, promoter stack, codon-optimization notes), the FBA predictions, computationally designed CRISPR guide RNAs, the datasheet, a short product paper, and a signed ZIP for provenance.