PepperSS-0087Heat LadderPepperProof of Concept

Verbal Warning

C. chinense · Heat Ladder: Verbal Warning

Scoville Heat Units

861,228

16M

5.4% of pure capsaicin

This is a computed design target (5.4% of pure-capsaicin concentration), derived from the flux model and blend chemistry — not a measured value. It sits within concentrations measured in real peppers. See the evidence behind it:

Paper & methods Datasheet Validation status How we know

Genes

Construct

13,345 bp

Variant

Triple

Promoter

2A11

Technical Manifest

Construct Type

T-DNA binary vector

Selection Marker

nptII (kanamycin resistance)

Codon Optimization

pepper

Capsaicinoid Profile

extreme multi

Gene Cassettes (8)

pAMT expression cassettevanillylamine biosynthesis

Pun1/CS (Triple) expression cassettecapsaicin synthase

BCAT expression cassettepathway enzyme

KAS expression cassettepathway enzyme

FAR expression cassettepathway enzyme

PAL expression cassettepathway enzyme

C4H expression cassettepathway enzyme

4CL expression cassettepathway enzyme

Construct map

13,345 bp · T-DNA binary vector

Left Border

nptII selection

pAMT expression

Pun1/CS expression

BCAT expression

KAS expression

FAR expression

PAL expression

C4H expression

4CL expression

Right Border

Left Border (LB) · 78 bp

nptII selection cassette · 795 bp

pAMT expression cassette

Pun1/CS (Triple) expression cassette

BCAT expression cassette

KAS expression cassette

FAR expression cassette

PAL expression cassette

C4H expression cassette

4CL expression cassette

Right Border (RB) · 75 bp

Burn Profile

Heat Ladder: Verbal Warning

Capsaicinoid blend: extreme multi

Onset 5s

Peak 45s

Duration 12m

Profile Full Spectrum

Specimen library

Organism documentation

On-plant

Cross-section

Clinical specimen

Download

This is one of our 10 founding pepper constructs — the proof of concept for the entire Scoville Splice platform. The full package is yours: academic paper, FASTA construct, datasheet, manifest, FBA predictions, CRISPR guides, and quick-start protocol.

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Read Full Paper →View Datasheet →Analyze in Platform →

Common questions

What is the target SHU of Verbal Warning?

Verbal Warning is engineered to a target of 861,228 Scoville Heat Units. Burn character: Heat Ladder: Verbal Warning. Capsaicinoid profile: extreme multi.

What host organism does Verbal Warning use?

Verbal Warning is a Pepper cultivar in the Heat Ladder series, built on Pepper (C. chinense). The construct runs 8 genes across 13,345 base pairs, driven by the 2A11 promoter system.

What license tiers are available for Verbal Warning?

Every catalog cultivar can be licensed at Specimen (one SKU, non-exclusive) or Sector (industry-sector exclusive) tiers. Genome and Platform licensing are available on request for category or stack-wide deals. See the licensing page for pricing structure.

What is included in the Verbal Warning download package?

The Verbal Warning bundle contains the full construct FASTA, a JSON manifest (gene list, promoter stack, codon-optimization notes), the FBA predictions, computationally designed CRISPR guide RNAs, the datasheet, a short product paper, and a signed ZIP for provenance.