Tier 2SS-0276NovaGround Cherry

Spark

P. peruviana · Berry heat

Scoville Heat Units

150

16M

0.0% of pure capsaicin

This is a computed design target (0.0% of pure-capsaicin concentration), derived from the flux model and blend chemistry — not a measured value. It sits within concentrations measured in real peppers. See the evidence behind it:

Paper & methods Datasheet Validation status How we know

Genes

Construct

6,477 bp

Variant

Promoter

E8 homolog

Technical Manifest

Construct Type

T-DNA binary vector

Selection Marker

nptII (kanamycin resistance)

Codon Optimization

solanaceae

Capsaicinoid Profile

Standard

Gene Cassettes (3)

pAMT expression cassettevanillylamine biosynthesis

Pun1/CS (WT) expression cassettecapsaicin synthase

BCAT expression cassettepathway enzyme

Construct map

6,477 bp · T-DNA binary vector

Left Border

nptII selection

pAMT expression

Pun1/CS expression

BCAT expression

Right Border

Left Border (LB) · 78 bp

nptII selection cassette · 795 bp

pAMT expression cassette

Pun1/CS (WT) expression cassette

BCAT expression cassette

Right Border (RB) · 75 bp

Burn Profile

Berry heat

Onset 4s

Peak 18s

Duration 2m

Profile Whisper

Specimen library

Organism documentation

On-plant

Cross-section

Clinical specimen

About this design

Spark is an engineered P. peruviana construct targeting 150 SHU — a Nova Series design in the Tier 2 tier. Berry heat

The construct is 6,477 bp, carrying 3 gene cassettes. T-DNA binary vector with solanaceae-native codon optimization. Selection marker: nptII (kanamycin resistance).

Package contents

FASTA constructAnnotated full-length DNA sequence (6,477 bp) with all cassette boundaries, promoter elements, and selection markers labeled.
One-page datasheetVisual lab reference: SHU target, gene cassette diagram, key parameters, and capsaicinoid blend ratio.
Technical manifestMachine-readable JSON with full construct specification: component list, selection marker, codon optimization, and capsaicinoid profile.
Flux balance analysisComplete FBA model output underlying the SHU design target: pathway flux predictions, reaction constraints, and metabolic network assumptions.
CRISPR guide designsGuide RNA sequences for cassette integration loci, with Tm values, GC content, off-target risk scores, and integration confirmation primers.
Plasmid mapCircular diagram, proportionally scaled, with all components labeled by position and function.
Transformation protocolStep-by-step guide for introducing this construct into P. peruviana: selection conditions, tissue preparation, and PCR-based screening strategy.

Delivered instantly

From $300

How to use this

This construct is packaged for introduction into P. peruviana. The transformation protocol covers the complete procedure for this host:

1
Prepare the expression system
This construct is packaged as a T-DNA binary vector for introduction into P. peruviana. See the transformation protocol for delivery method and host preparation details.
2
Transform Ground Cherry
Introduce the construct using the method specified in the protocol. Delivery conditions are specific to P. peruviana tissue competency and the construct's integration requirements.
3
Select with nptII (kanamycin resistance)
Propagate on selection medium at conditions given in the protocol. Non-transformed lines will not survive.
4
Screen and advance
Confirm integration using the primers included in the CRISPR guide designs. Advance confirmed transformants to the appropriate developmental stage to assess capsaicinoid expression.

License this cultivar

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Take it further with CRISPR

The CRISPR guide designs in this package are ready to use with Cas9 or Cpf1 for precision integration at defined genomic loci — and for disrupting competing pathway enzymes that reduce capsaicinoid yield. For site-specific insertion, pathway stacking, or multiplexed editing across ground cherry cultivars, the guides are your starting point.

Read the full paper →View license tiers →Custom CRISPR engagement →

Work with Scoville Splice

This construct is a research tool. What it can become is an ingredient.

Scoville Splice works with food and beverage companies to take engineered capsaicinoid constructs from sequence to supply chain. If you’re developing a product — a sauce, a seasoning, a functional food — and you need a precise, consistent heat profile that no natural ground cherry can deliver, that’s what we were built for. We offer custom cultivar development, strain characterization, pilot-scale grow protocols, and formulation consulting.

Already licensed?

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Common questions

What is the target SHU of Spark?

Spark is engineered to a target of 150 Scoville Heat Units. Burn character: Berry heat. Capsaicinoid profile: -.

What host organism does Spark use?

Spark is a Tier 2 cultivar in the Nova series, built on Ground Cherry (P. peruviana). The construct runs 3 genes across 6,477 base pairs, driven by the E8 homolog promoter system.

What license tiers are available for Spark?

Every catalog cultivar can be licensed at Specimen (one SKU, non-exclusive) or Sector (industry-sector exclusive) tiers. Genome and Platform licensing are available on request for category or stack-wide deals. See the licensing page for pricing structure.

What is included in the Spark download package?

The Spark bundle contains the full construct FASTA, a JSON manifest (gene list, promoter stack, codon-optimization notes), the FBA predictions, computationally designed CRISPR guide RNAs, the datasheet, a short product paper, and a signed ZIP for provenance.