PepperSS-0189ReservePepper

Collaboration No. 1

C. chinense · Reserve: Collaboration No. 1

Scoville Heat Units

29,929

16M

0.2% of pure capsaicin

This is a computed design target (0.2% of pure-capsaicin concentration), derived from the flux model and blend chemistry — not a measured value. It sits within concentrations measured in real peppers. See the evidence behind it:

Paper & methods Datasheet Validation status How we know

Genes

Construct

9,181 bp

Variant

S39L+L345G

Promoter

2A11

Technical Manifest

Construct Type

T-DNA binary vector

Selection Marker

nptII (kanamycin resistance)

Codon Optimization

pepper

Capsaicinoid Profile

reserve

Gene Cassettes (5)

pAMT expression cassettevanillylamine biosynthesis

Pun1/CS (S39L+L345G) expression cassettecapsaicin synthase

BCAT expression cassettepathway enzyme

KAS expression cassettepathway enzyme

FAR expression cassettepathway enzyme

Construct map

9,181 bp · T-DNA binary vector

Left Border

nptII selection

pAMT expression

Pun1/CS expression

BCAT expression

KAS expression

FAR expression

Right Border

Left Border (LB) · 78 bp

nptII selection cassette · 795 bp

pAMT expression cassette

Pun1/CS (S39L+L345G) expression cassette

BCAT expression cassette

KAS expression cassette

FAR expression cassette

Right Border (RB) · 75 bp

Burn Profile

Reserve: Collaboration No. 1

Capsaicinoid blend: reserve

Onset 4s

Peak 18s

Duration 2m

Profile Whisper

Specimen library

Organism documentation

On-plant

Cross-section

Clinical specimen

About this design

Collaboration No. 1 is an engineered C. chinense construct targeting 29.9K SHU — a Reserve Series design in the Pepper tier. Reserve: Collaboration No. 1

The construct is 9,181 bp, carrying 5 gene cassettes. T-DNA binary vector with pepper-native codon optimization. Selection marker: nptII (kanamycin resistance). Capsaicinoid profile: reserve.

Package contents

FASTA constructAnnotated full-length DNA sequence (9,181 bp) with all cassette boundaries, promoter elements, and selection markers labeled.
One-page datasheetVisual lab reference: SHU target, gene cassette diagram, key parameters, and capsaicinoid blend ratio.
Technical manifestMachine-readable JSON with full construct specification: component list, selection marker, codon optimization, and capsaicinoid profile.
Flux balance analysisComplete FBA model output underlying the SHU design target: pathway flux predictions, reaction constraints, and metabolic network assumptions.
CRISPR guide designsGuide RNA sequences for cassette integration loci, with Tm values, GC content, off-target risk scores, and integration confirmation primers.
Plasmid mapCircular diagram, proportionally scaled, with all components labeled by position and function.
Transformation protocolStep-by-step guide for introducing this construct into C. chinense: selection conditions, tissue preparation, and PCR-based screening strategy.

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How to use this

This is a T-DNA binary vector for Agrobacterium-mediated transformation of C. chinense. Path from this package to a living plant:

1
Transform Agrobacterium
Introduce the construct into Agrobacterium tumefaciens (strain GV3101 or LBA4404) by electroporation or freeze-thaw.
2
Inoculate pepper explants
Co-cultivate with leaf discs or cotyledon sections from C. chinense seedlings at the 4–6 leaf stage.
3
Select on nptII (kanamycin resistance)
Non-transformed tissue will not survive. Run selection on MS medium at conditions specified in the protocol.
4
Screen regenerants by PCR
Use the integration confirmation primers from the CRISPR guide designs file to verify cassette insertion before advancing to T1 generation.
5
Grow out and measure
Advance confirmed transgenic lines to fruit-bearing stage. Confirm capsaicinoid content by HPLC or capsaicin-specific colorimetric assay.

License this cultivar

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Take it further with CRISPR

The CRISPR guide designs in this package are ready to use with Cas9 or Cpf1 for precision integration at defined genomic loci — and for disrupting competing pathway enzymes that reduce capsaicinoid yield. For site-specific insertion, pathway stacking, or multiplexed editing across pepper cultivars, the guides are your starting point.

Read the full paper →View license tiers →Custom CRISPR engagement →

Work with Scoville Splice

This construct is a research tool. What it can become is an ingredient.

Scoville Splice works with food and beverage companies to take engineered capsaicinoid constructs from sequence to supply chain. If you’re developing a product — a sauce, a seasoning, a functional food — and you need a precise, consistent heat profile that no natural pepper can deliver, that’s what we were built for. We offer custom cultivar development, strain characterization, pilot-scale grow protocols, and formulation consulting.

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Common questions

What is the target SHU of Collaboration No. 1?

Collaboration No. 1 is engineered to a target of 29,929 Scoville Heat Units. Burn character: Reserve: Collaboration No. 1. Capsaicinoid profile: reserve.

What host organism does Collaboration No. 1 use?

Collaboration No. 1 is a Pepper cultivar in the Reserve series, built on Pepper (C. chinense). The construct runs 5 genes across 9,181 base pairs, driven by the 2A11 promoter system.

What license tiers are available for Collaboration No. 1?

Every catalog cultivar can be licensed at Specimen (one SKU, non-exclusive) or Sector (industry-sector exclusive) tiers. Genome and Platform licensing are available on request for category or stack-wide deals. See the licensing page for pricing structure.

What is included in the Collaboration No. 1 download package?

The Collaboration No. 1 bundle contains the full construct FASTA, a JSON manifest (gene list, promoter stack, codon-optimization notes), the FBA predictions, computationally designed CRISPR guide RNAs, the datasheet, a short product paper, and a signed ZIP for provenance.