PepperSS-0129TerroirPepper

Bhut Lineage

C. chinense · Terroir: Bhut Lineage

Scoville Heat Units

149,646

16M

0.9% of pure capsaicin

This is a computed design target (0.9% of pure-capsaicin concentration), derived from the flux model and blend chemistry — not a measured value. It sits within concentrations measured in real peppers. See the evidence behind it:

Paper & methods Datasheet Validation status How we know

Genes

Construct

12,026 bp

Variant

Triple

Promoter

2A11

Technical Manifest

Construct Type

T-DNA binary vector

Selection Marker

nptII (kanamycin resistance)

Codon Optimization

pepper

Capsaicinoid Profile

C. chinense

Gene Cassettes (7)

pAMT expression cassettevanillylamine biosynthesis

Pun1/CS (Triple) expression cassettecapsaicin synthase

BCAT expression cassettepathway enzyme

KAS expression cassettepathway enzyme

FAR expression cassettepathway enzyme

PAL expression cassettepathway enzyme

C4H expression cassettepathway enzyme

Construct map

12,026 bp · T-DNA binary vector

Left Border

nptII selection

pAMT expression

Pun1/CS expression

BCAT expression

KAS expression

FAR expression

PAL expression

C4H expression

Right Border

Left Border (LB) · 78 bp

nptII selection cassette · 795 bp

pAMT expression cassette

Pun1/CS (Triple) expression cassette

BCAT expression cassette

KAS expression cassette

FAR expression cassette

PAL expression cassette

C4H expression cassette

Right Border (RB) · 75 bp

Burn Profile

Terroir: Bhut Lineage

Capsaicinoid blend: C. chinense

Onset 20s

Peak 2m

Duration 12m

Profile Afterglow

Specimen library

Organism documentation

On-plant

Cross-section

Clinical specimen

About this design

Bhut Lineage is an engineered C. chinense construct targeting 149.6K SHU — a Terroir Series design in the Pepper tier. Terroir: Bhut Lineage

The construct is 12,026 bp, carrying 7 gene cassettes. T-DNA binary vector with pepper-native codon optimization. Selection marker: nptII (kanamycin resistance). Capsaicinoid profile: C. chinense.

Package contents

FASTA constructAnnotated full-length DNA sequence (12,026 bp) with all cassette boundaries, promoter elements, and selection markers labeled.
One-page datasheetVisual lab reference: SHU target, gene cassette diagram, key parameters, and capsaicinoid blend ratio.
Technical manifestMachine-readable JSON with full construct specification: component list, selection marker, codon optimization, and capsaicinoid profile.
Flux balance analysisComplete FBA model output underlying the SHU design target: pathway flux predictions, reaction constraints, and metabolic network assumptions.
CRISPR guide designsGuide RNA sequences for cassette integration loci, with Tm values, GC content, off-target risk scores, and integration confirmation primers.
Plasmid mapCircular diagram, proportionally scaled, with all components labeled by position and function.
Transformation protocolStep-by-step guide for introducing this construct into C. chinense: selection conditions, tissue preparation, and PCR-based screening strategy.

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How to use this

This is a T-DNA binary vector for Agrobacterium-mediated transformation of C. chinense. Path from this package to a living plant:

1
Transform Agrobacterium
Introduce the construct into Agrobacterium tumefaciens (strain GV3101 or LBA4404) by electroporation or freeze-thaw.
2
Inoculate pepper explants
Co-cultivate with leaf discs or cotyledon sections from C. chinense seedlings at the 4–6 leaf stage.
3
Select on nptII (kanamycin resistance)
Non-transformed tissue will not survive. Run selection on MS medium at conditions specified in the protocol.
4
Screen regenerants by PCR
Use the integration confirmation primers from the CRISPR guide designs file to verify cassette insertion before advancing to T1 generation.
5
Grow out and measure
Advance confirmed transgenic lines to fruit-bearing stage. Confirm capsaicinoid content by HPLC or capsaicin-specific colorimetric assay.

License this cultivar

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Take it further with CRISPR

The CRISPR guide designs in this package are ready to use with Cas9 or Cpf1 for precision integration at defined genomic loci — and for disrupting competing pathway enzymes that reduce capsaicinoid yield. For site-specific insertion, pathway stacking, or multiplexed editing across pepper cultivars, the guides are your starting point.

Read the full paper →View license tiers →Custom CRISPR engagement →

Work with Scoville Splice

This construct is a research tool. What it can become is an ingredient.

Scoville Splice works with food and beverage companies to take engineered capsaicinoid constructs from sequence to supply chain. If you’re developing a product — a sauce, a seasoning, a functional food — and you need a precise, consistent heat profile that no natural pepper can deliver, that’s what we were built for. We offer custom cultivar development, strain characterization, pilot-scale grow protocols, and formulation consulting.

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Common questions

What is the target SHU of Bhut Lineage?

Bhut Lineage is engineered to a target of 149,646 Scoville Heat Units. Burn character: Terroir: Bhut Lineage. Capsaicinoid profile: C. chinense.

What host organism does Bhut Lineage use?

Bhut Lineage is a Pepper cultivar in the Terroir series, built on Pepper (C. chinense). The construct runs 7 genes across 12,026 base pairs, driven by the 2A11 promoter system.

What license tiers are available for Bhut Lineage?

Every catalog cultivar can be licensed at Specimen (one SKU, non-exclusive) or Sector (industry-sector exclusive) tiers. Genome and Platform licensing are available on request for category or stack-wide deals. See the licensing page for pricing structure.

What is included in the Bhut Lineage download package?

The Bhut Lineage bundle contains the full construct FASTA, a JSON manifest (gene list, promoter stack, codon-optimization notes), the FBA predictions, computationally designed CRISPR guide RNAs, the datasheet, a short product paper, and a signed ZIP for provenance.