PepperSS-0105SpectrumPepper

Afterglow

C. annuum · Spectrum: Afterglow

Scoville Heat Units

1,497

16M

0.0% of pure capsaicin

This is a computed design target (0.0% of pure-capsaicin concentration), derived from the flux model and blend chemistry — not a measured value. It sits within concentrations measured in real peppers. See the evidence behind it:

Paper & methods Datasheet Validation status How we know

Genes

Construct

4,852 bp

Variant

Promoter

Technical Manifest

Construct Type

T-DNA binary vector

Selection Marker

nptII (kanamycin resistance)

Codon Optimization

pepper

Capsaicinoid Profile

burn: Afterglow

Gene Cassettes (2)

pAMT expression cassettevanillylamine biosynthesis

Pun1/CS (WT) expression cassettecapsaicin synthase

Construct map

4,852 bp · T-DNA binary vector

Left Border

nptII selection

pAMT expression

Pun1/CS expression

Right Border

Left Border (LB) · 78 bp

nptII selection cassette · 795 bp

pAMT expression cassette

Pun1/CS (WT) expression cassette

Right Border (RB) · 75 bp

Burn Profile

Spectrum: Afterglow

Capsaicinoid blend: Afterglow

Onset 20s

Peak 2m

Duration 12m

Profile Afterglow

Specimen library

Organism documentation

On-plant

Cross-section

Clinical specimen

About this design

Afterglow is an engineered C. annuum construct targeting 1.5K SHU — a Spectrum Series design in the Pepper tier. Spectrum: Afterglow

The construct is 4,852 bp, carrying 2 gene cassettes. T-DNA binary vector with pepper-native codon optimization. Selection marker: nptII (kanamycin resistance). Capsaicinoid profile: burn: Afterglow.

Package contents

FASTA constructAnnotated full-length DNA sequence (4,852 bp) with all cassette boundaries, promoter elements, and selection markers labeled.
One-page datasheetVisual lab reference: SHU target, gene cassette diagram, key parameters, and capsaicinoid blend ratio.
Technical manifestMachine-readable JSON with full construct specification: component list, selection marker, codon optimization, and capsaicinoid profile.
Flux balance analysisComplete FBA model output underlying the SHU design target: pathway flux predictions, reaction constraints, and metabolic network assumptions.
CRISPR guide designsGuide RNA sequences for cassette integration loci, with Tm values, GC content, off-target risk scores, and integration confirmation primers.
Plasmid mapCircular diagram, proportionally scaled, with all components labeled by position and function.
Transformation protocolStep-by-step guide for introducing this construct into C. annuum: selection conditions, tissue preparation, and PCR-based screening strategy.

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How to use this

This is a T-DNA binary vector for Agrobacterium-mediated transformation of C. annuum. Path from this package to a living plant:

1
Transform Agrobacterium
Introduce the construct into Agrobacterium tumefaciens (strain GV3101 or LBA4404) by electroporation or freeze-thaw.
2
Inoculate pepper explants
Co-cultivate with leaf discs or cotyledon sections from C. annuum seedlings at the 4–6 leaf stage.
3
Select on nptII (kanamycin resistance)
Non-transformed tissue will not survive. Run selection on MS medium at conditions specified in the protocol.
4
Screen regenerants by PCR
Use the integration confirmation primers from the CRISPR guide designs file to verify cassette insertion before advancing to T1 generation.
5
Grow out and measure
Advance confirmed transgenic lines to fruit-bearing stage. Confirm capsaicinoid content by HPLC or capsaicin-specific colorimetric assay.

License this cultivar

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Take it further with CRISPR

The CRISPR guide designs in this package are ready to use with Cas9 or Cpf1 for precision integration at defined genomic loci — and for disrupting competing pathway enzymes that reduce capsaicinoid yield. For site-specific insertion, pathway stacking, or multiplexed editing across pepper cultivars, the guides are your starting point.

Read the full paper →View license tiers →Custom CRISPR engagement →

Work with Scoville Splice

This construct is a research tool. What it can become is an ingredient.

Scoville Splice works with food and beverage companies to take engineered capsaicinoid constructs from sequence to supply chain. If you’re developing a product — a sauce, a seasoning, a functional food — and you need a precise, consistent heat profile that no natural pepper can deliver, that’s what we were built for. We offer custom cultivar development, strain characterization, pilot-scale grow protocols, and formulation consulting.

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Common questions

What is the target SHU of Afterglow?

Afterglow is engineered to a target of 1,497 Scoville Heat Units. Burn character: Spectrum: Afterglow. Capsaicinoid profile: burn: Afterglow.

What host organism does Afterglow use?

Afterglow is a Pepper cultivar in the Spectrum series, built on Pepper (C. annuum). The construct runs 2 genes across 4,852 base pairs, driven by the E8 promoter system.

What license tiers are available for Afterglow?

Every catalog cultivar can be licensed at Specimen (one SKU, non-exclusive) or Sector (industry-sector exclusive) tiers. Genome and Platform licensing are available on request for category or stack-wide deals. See the licensing page for pricing structure.

What is included in the Afterglow download package?

The Afterglow bundle contains the full construct FASTA, a JSON manifest (gene list, promoter stack, codon-optimization notes), the FBA predictions, computationally designed CRISPR guide RNAs, the datasheet, a short product paper, and a signed ZIP for provenance.