Zero-Bitter Brassica: TGG1/TGG2 Myrosinase Knockout for IFI-B Construct Specifications
Priyanca Ford · ORCID 0000-0003-0395-1752 · Scoville Splice LLC · 2026
Abstract
Elimination of glucosinolate-derived bitterness in Brassica species via CRISPR-SpCas9 knockout of TGG1 and TGG2 (thioglucoside glucohydrolases / myrosinases), preventing hydrolysis of glucosinolates into bitter isothiocyanates, nitriles, and epithionitriles. Demonstrated across broccoli, Brussels sprouts, and kale. Construct specifications target TAS2R38 suppression for consumer and commercial bitterness reduction applications. PDF and full specifications in preparation.
Keywords
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TGG1/TGG2 knockout construct sequences (pending accession)
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