Umami-Enriched Capsicum via GAD1/4 Knockout and GDH1 Overexpression: IFI-U Construct Specifications
Priyanca Ford · ORCID 0000-0003-0395-1752 · Scoville Splice LLC · 2026
Abstract
Engineering free glutamate accumulation in Capsicum through knockout of GAD1 and GAD4 (glutamate decarboxylases that consume free glutamate) combined with overexpression of GDH1 (glutamate dehydrogenase for increased biosynthesis). Achieves TAS1R1/TAS1R3 umami receptor activation profiles measurable on the IFI-U sub-scale. Construct specifications target 200 consumer and 1,000 commercial product formulations. PDF and full specifications in preparation.
Keywords
Repository & Data Links
GAD1/4 knockout + GDH1 OE construct sequences (pending accession)
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