TRPA1 Spectrum Engineering: CrCCR1 Cinnamaldehyde Pathway for IFI-P Pungency Construct Specifications

Engineering TRPA1 receptor activation through heterologous expression of CrCCR1 (cinnamoyl-CoA reductase) and complementary enzymes for cinnamaldehyde biosynthesis. TRPA1 is activated by a broader spectrum of electrophilic compounds than TRPV1, enabling novel pungency profiles not achievable with capsaicinoids alone. Construct specifications target the IFI-P pungency sub-scale for applications in spice crops, condiments, and controlled-pungency products. PDF and full specifications in preparation.