Synergy Index V-IV-B

Technical Specifications

Pathway Edits

• ▸SlGDH1 and SlGOGAT simultaneous upregulation via bidirectional synthetic promoter construct for coupled glutamate flux amplification
• ▸Adenylosuccinate lyase SlADSL pathway boost for AMP accumulation as synergistic TAS1R1/TAS1R3 co-ligand
• ▸IMP dehydrogenase SlIMPDH targeted knockdown (70% via amiRNA) to preserve IMP pool from conversion to XMP
• ▸Cytosolic nucleotide transporter SlENT1 upregulation for enhanced cytosolic 5-nucleotide availability
• ▸5-GMP synthase from Aspergillus niger heterologous expression for GMP co-accumulation in pericarp tissue
• ▸SlAAT2 aspartate aminotransferase overexpression for enhanced Asp-to-Glu carbon routing under TCA surplus
• ▸Feedback-resistant SlGS2 mutant (R297A, K299E) for plastidic glutamine synthetase desensitization
• ▸SlVGT3 tonoplast glutamate transporter overexpression for controlled vacuolar buffering and cytosolic pool maintenance
• ▸SlNRT2.1 nitrate transporter upregulation for enhanced inorganic nitrogen supply to glutamate biosynthesis
• ▸SlGln1;1 overexpression under senescence-induced promoter for late-stage glutamate accumulation peak
• ▸SlPK1 pyruvate kinase allosteric variant introduction to redirect phosphoenolpyruvate toward TCA over glycolysis
• ▸SlFUM1 fumarase upregulation to maximize malate-fumarate cycle feeding into OAA-glutamate pathway
• ▸SlCS1 citrate synthase partial knockdown to reduce competitive OAA withdrawal into full TCA cycle
• ▸SlMDH1 malate dehydrogenase overexpression for enhanced malate-to-OAA conversion at TCA-glutamate junction
• ▸SlIDH2 isocitrate dehydrogenase NADP-dependent isoform upregulation for alpha-ketoglutarate maximal supply
• ▸SlGS1;3 root-specific isoform ectopic overexpression in pericarp under 2A11 promoter for added GS coverage

Key Interventions