Specimen SS-F-UMA-176 // UMAMI · IFI-U
Synergy Index II-IV-B
Solanum lycopersicum
Tomato
Target Trait
7.76×
fold-change
Nucleotide-glutamate synergy ratio
Technical Specifications
Genomic Edits
10
GC Content
43-49%
Construct Length
2,400 bp
Promoter System
T7/SP6 cell-free
Expression System
In-vitro cell-free
Host Strain
Cell-free
Receptor Target
TAS1R1/TAS1R3 + mGluR1
IFI Score
659.6
Pathway Edits
- SlGDH1 and SlGOGAT simultaneous upregulation via bidirectional synthetic promoter construct for coupled glutamate flux amplification
- Adenylosuccinate lyase SlADSL pathway boost for AMP accumulation as synergistic TAS1R1/TAS1R3 co-ligand
- IMP dehydrogenase SlIMPDH targeted knockdown (70% via amiRNA) to preserve IMP pool from conversion to XMP
- Cytosolic nucleotide transporter SlENT1 upregulation for enhanced cytosolic 5-nucleotide availability
- 5-GMP synthase from Aspergillus niger heterologous expression for GMP co-accumulation in pericarp tissue
- SlAAT2 aspartate aminotransferase overexpression for enhanced Asp-to-Glu carbon routing under TCA surplus
- Feedback-resistant SlGS2 mutant (R297A, K299E) for plastidic glutamine synthetase desensitization
- SlVGT3 tonoplast glutamate transporter overexpression for controlled vacuolar buffering and cytosolic pool maintenance
- SlNRT2.1 nitrate transporter upregulation for enhanced inorganic nitrogen supply to glutamate biosynthesis
- SlGln1;1 overexpression under senescence-induced promoter for late-stage glutamate accumulation peak
Key Interventions
IFI Subscale Profile
IFI Profile
Use Case
Premium umami concentrates, high-end food service applications, and nutraceutical umami modulators. Flagship series targeting the highest documented IFI-U scores achievable through dual-nucleotide and glutamate co-engineering.
License
$149 · Tier II License
Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.
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