IFI-U · Synergy Index

Specimen SS-F-UMA-174 // UMAMI · IFI-U

Synergy Index II-PR-B

Solanum lycopersicum

Tomato

Tier IISynergy Index

Target Trait

7.76×

fold-change

Nucleotide-glutamate synergy ratio

Technical Specifications

Genomic Edits

10

GC Content

43-49%

Construct Length

2,400 bp

Promoter System

Ubiquitin / T7

Expression System

Protoplast PEG-fusion

Host Strain

PEG-mediated

Receptor Target

TAS1R1/TAS1R3 + mGluR1

IFI Score

659.6

Pathway Edits

  • SlGDH1 and SlGOGAT simultaneous upregulation via bidirectional synthetic promoter construct for coupled glutamate flux amplification
  • Adenylosuccinate lyase SlADSL pathway boost for AMP accumulation as synergistic TAS1R1/TAS1R3 co-ligand
  • IMP dehydrogenase SlIMPDH targeted knockdown (70% via amiRNA) to preserve IMP pool from conversion to XMP
  • Cytosolic nucleotide transporter SlENT1 upregulation for enhanced cytosolic 5-nucleotide availability
  • 5-GMP synthase from Aspergillus niger heterologous expression for GMP co-accumulation in pericarp tissue
  • SlAAT2 aspartate aminotransferase overexpression for enhanced Asp-to-Glu carbon routing under TCA surplus
  • Feedback-resistant SlGS2 mutant (R297A, K299E) for plastidic glutamine synthetase desensitization
  • SlVGT3 tonoplast glutamate transporter overexpression for controlled vacuolar buffering and cytosolic pool maintenance
  • SlNRT2.1 nitrate transporter upregulation for enhanced inorganic nitrogen supply to glutamate biosynthesis
  • SlGln1;1 overexpression under senescence-induced promoter for late-stage glutamate accumulation peak

Key Interventions

Synergistic 5-nucleotide + glutamate co-engineering targeting maximum TAS1R1/TAS1R3 receptor occupancy by both ligand classes simultaneouslyTAS1R1/TAS1R3 maximum co-ligand occupancy achieved via coupled IMP/GMP and free glutamate peak alignmentmGluR1 positive allosteric modulation via elevated synaptic-analog glutamate concentrations in tissue matrix

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
659.6
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

Premium umami concentrates, high-end food service applications, and nutraceutical umami modulators. Flagship series targeting the highest documented IFI-U scores achievable through dual-nucleotide and glutamate co-engineering.

License

$149 · Tier II License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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