Broth Depth V-PR-A

Technical Specifications

Pathway Edits

• ▸GmGS1a and GmGS1b cytosolic isoform co-overexpression under seed-specific beta-conglycinin promoter
• ▸GmGS2 plastidic isoform overexpression under CaMV35S for vegetative tissue coverage
• ▸GmPEPC1 downregulation via RNAi to block OAA diversion from TCA-glutamate interface
• ▸GmAAT1 aspartate aminotransferase allosteric variant for enhanced glutamate production over aspartate
• ▸GmSSADH succinate-semialdehyde dehydrogenase suppression to minimize GABA shunt flux away from glutamate
• ▸GmVGT2 vacuolar glutamate transporter upregulation for cytosolic glutamate maintenance under metabolic load
• ▸GmLOX1 lipoxygenase partial suppression (50% via miRNA) to reduce competing off-note aldehydes that mask umami
• ▸GmNADP-ME NADP-dependent malic enzyme upregulation for malate-to-OAA-to-glutamate carbon cycling amplification
• ▸GmGDH heterologous expression from Glycine soja for expanded catabolic glutamate dehydrogenase capacity
• ▸GmAlaAT alanine aminotransferase redirection variant for pyruvate flux toward TCA rather than alanine accumulation
• ▸GmCPS carbamoyl phosphate synthetase partial knockdown to reduce urea cycle competition for glutamate carbon
• ▸GmPro1 proline biosynthesis suppression via P5CS RNAi to redirect glutamate-semialdehyde back to free glutamate
• ▸GmAsn1 asparagine synthetase downregulation to prevent glutamine diversion from glutamate pool
• ▸GmFDX2 ferredoxin upregulation for enhanced GOGAT electron supply in chloroplast-glutamate synthesis

Key Interventions