Specimen SS-F-UMA-141 // UMAMI · IFI-U
Broth Depth III-PR-A
Glycine max
Soybean
Target Trait
7×
fold-change
Broth umami depth index
Technical Specifications
Genomic Edits
10
GC Content
44-50%
Construct Length
2,800 bp
Promoter System
Ubiquitin / T7
Expression System
Protoplast PEG-fusion
Host Strain
PEG-mediated
Receptor Target
TAS1R1/TAS1R3 + mGluR1
IFI Score
595
Pathway Edits
- GmGS1a and GmGS1b cytosolic isoform co-overexpression under seed-specific beta-conglycinin promoter
- GmGS2 plastidic isoform overexpression under CaMV35S for vegetative tissue coverage
- GmPEPC1 downregulation via RNAi to block OAA diversion from TCA-glutamate interface
- GmAAT1 aspartate aminotransferase allosteric variant for enhanced glutamate production over aspartate
- GmSSADH succinate-semialdehyde dehydrogenase suppression to minimize GABA shunt flux away from glutamate
- GmVGT2 vacuolar glutamate transporter upregulation for cytosolic glutamate maintenance under metabolic load
- GmLOX1 lipoxygenase partial suppression (50% via miRNA) to reduce competing off-note aldehydes that mask umami
- GmNADP-ME NADP-dependent malic enzyme upregulation for malate-to-OAA-to-glutamate carbon cycling amplification
- GmGDH heterologous expression from Glycine soja for expanded catabolic glutamate dehydrogenase capacity
- GmAlaAT alanine aminotransferase redirection variant for pyruvate flux toward TCA rather than alanine accumulation
Key Interventions
IFI Subscale Profile
IFI Profile
Use Case
Soy-based broths, dashi analogs, plant-based meat flavor systems, and umami seasoning blends. Engineered for applications where deep, sustained umami with clean finish is the primary functional requirement.
License
$149 · Tier III License
Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.
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