Specimen SS-F-UMA-130 // UMAMI · IFI-U
Broth Depth II-AG-B
Glycine max
Soybean
Target Trait
6.32×
fold-change
Broth umami depth index
Technical Specifications
Genomic Edits
9
GC Content
43-49%
Construct Length
2,320 bp
Promoter System
Tissue-specific 2A11
Expression System
Agrobacterium-mediated T-DNA
Host Strain
EHA105
Receptor Target
TAS1R1/TAS1R3 + mGluR1
IFI Score
537.2
Pathway Edits
- GmGS1a and GmGS1b cytosolic isoform co-overexpression under seed-specific beta-conglycinin promoter
- GmGS2 plastidic isoform overexpression under CaMV35S for vegetative tissue coverage
- GmPEPC1 downregulation via RNAi to block OAA diversion from TCA-glutamate interface
- GmAAT1 aspartate aminotransferase allosteric variant for enhanced glutamate production over aspartate
- GmSSADH succinate-semialdehyde dehydrogenase suppression to minimize GABA shunt flux away from glutamate
- GmVGT2 vacuolar glutamate transporter upregulation for cytosolic glutamate maintenance under metabolic load
- GmLOX1 lipoxygenase partial suppression (50% via miRNA) to reduce competing off-note aldehydes that mask umami
- GmNADP-ME NADP-dependent malic enzyme upregulation for malate-to-OAA-to-glutamate carbon cycling amplification
- GmGDH heterologous expression from Glycine soja for expanded catabolic glutamate dehydrogenase capacity
Key Interventions
IFI Subscale Profile
IFI Profile
Use Case
Soy-based broths, dashi analogs, plant-based meat flavor systems, and umami seasoning blends. Engineered for applications where deep, sustained umami with clean finish is the primary functional requirement.
License
$149 · Tier II License
Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.
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