IFI-U · Broth Depth

Specimen SS-F-UMA-129 // UMAMI · IFI-U

Broth Depth II-AG-A

Glycine max

Soybean

Tier IIBroth Depth

Target Trait

5.5×

fold-change

Broth umami depth index

Technical Specifications

Genomic Edits

8

GC Content

43-49%

Construct Length

2,240 bp

Promoter System

Tissue-specific 2A11

Expression System

Agrobacterium-mediated T-DNA

Host Strain

EHA105

Receptor Target

TAS1R1/TAS1R3 + mGluR1

IFI Score

467.5

Pathway Edits

  • GmGS1a and GmGS1b cytosolic isoform co-overexpression under seed-specific beta-conglycinin promoter
  • GmGS2 plastidic isoform overexpression under CaMV35S for vegetative tissue coverage
  • GmPEPC1 downregulation via RNAi to block OAA diversion from TCA-glutamate interface
  • GmAAT1 aspartate aminotransferase allosteric variant for enhanced glutamate production over aspartate
  • GmSSADH succinate-semialdehyde dehydrogenase suppression to minimize GABA shunt flux away from glutamate
  • GmVGT2 vacuolar glutamate transporter upregulation for cytosolic glutamate maintenance under metabolic load
  • GmLOX1 lipoxygenase partial suppression (50% via miRNA) to reduce competing off-note aldehydes that mask umami
  • GmNADP-ME NADP-dependent malic enzyme upregulation for malate-to-OAA-to-glutamate carbon cycling amplification

Key Interventions

Multi-axis glutamate biosynthesis enhancement spanning cytosolic, plastidic, and mitochondrial compartmentsCompeting flavor compound suppression targeting lipoxygenase-derived off-notes and GABA accumulationmGluR1 agonist profile enrichment via clean glutamate signal with reduced competing amino acid background

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
467.5
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

Soy-based broths, dashi analogs, plant-based meat flavor systems, and umami seasoning blends. Engineered for applications where deep, sustained umami with clean finish is the primary functional requirement.

License

$99 · Tier II License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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