IFI-U · Broth Depth

Specimen SS-F-UMA-127 // UMAMI · IFI-U

Broth Depth I-IV-A

Glycine max

Soybean

Tier IBroth Depth

Target Trait

4×

fold-change

Broth umami depth index

Technical Specifications

Genomic Edits

6

GC Content

42-48%

Construct Length

1,680 bp

Promoter System

T7/SP6 cell-free

Expression System

In-vitro cell-free

Host Strain

Cell-free

Receptor Target

TAS1R1/TAS1R3 + mGluR1

IFI Score

340

Pathway Edits

  • GmGS1a and GmGS1b cytosolic isoform co-overexpression under seed-specific beta-conglycinin promoter
  • GmGS2 plastidic isoform overexpression under CaMV35S for vegetative tissue coverage
  • GmPEPC1 downregulation via RNAi to block OAA diversion from TCA-glutamate interface
  • GmAAT1 aspartate aminotransferase allosteric variant for enhanced glutamate production over aspartate
  • GmSSADH succinate-semialdehyde dehydrogenase suppression to minimize GABA shunt flux away from glutamate
  • GmVGT2 vacuolar glutamate transporter upregulation for cytosolic glutamate maintenance under metabolic load

Key Interventions

Multi-axis glutamate biosynthesis enhancement spanning cytosolic, plastidic, and mitochondrial compartmentsCompeting flavor compound suppression targeting lipoxygenase-derived off-notes and GABA accumulationmGluR1 agonist profile enrichment via clean glutamate signal with reduced competing amino acid background

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
340
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

Soy-based broths, dashi analogs, plant-based meat flavor systems, and umami seasoning blends. Engineered for applications where deep, sustained umami with clean finish is the primary functional requirement.

License

$49 · Tier I License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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