Broth Depth I-AG-A

Glycine max

Soybean

Tier IBroth Depth

Target Trait

4×

fold-change

Broth umami depth index

Technical Specifications

Genomic Edits

GC Content

42-48%

Construct Length

1,680 bp

Promoter System

Tissue-specific 2A11

Expression System

Agrobacterium-mediated T-DNA

Host Strain

EHA105

Receptor Target

TAS1R1/TAS1R3 + mGluR1

IFI Score

340

Pathway Edits

GmGS1a and GmGS1b cytosolic isoform co-overexpression under seed-specific beta-conglycinin promoter
GmGS2 plastidic isoform overexpression under CaMV35S for vegetative tissue coverage
GmPEPC1 downregulation via RNAi to block OAA diversion from TCA-glutamate interface
GmAAT1 aspartate aminotransferase allosteric variant for enhanced glutamate production over aspartate
GmSSADH succinate-semialdehyde dehydrogenase suppression to minimize GABA shunt flux away from glutamate
GmVGT2 vacuolar glutamate transporter upregulation for cytosolic glutamate maintenance under metabolic load

Key Interventions

Multi-axis glutamate biosynthesis enhancement spanning cytosolic, plastidic, and mitochondrial compartmentsCompeting flavor compound suppression targeting lipoxygenase-derived off-notes and GABA accumulationmGluR1 agonist profile enrichment via clean glutamate signal with reduced competing amino acid background

IFI Subscale Profile

IFI Profile

IFI-S

—

IFI-U

340

IFI-B

—

IFI-K

—

IFI-C

—

IFI-P

—

IFI-A

—

IFI-F

—

Use Case

Soy-based broths, dashi analogs, plant-based meat flavor systems, and umami seasoning blends. Engineered for applications where deep, sustained umami with clean finish is the primary functional requirement.

License

$49 · Tier I License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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