MSG Equivalent V-IV-B

Technical Specifications

Pathway Edits

• ▸AbGDH overexpression under constitutive Agaricus gpd promoter for alpha-ketoglutarate to glutamate maximal flux
• ▸Inosine 5-monophosphate synthase AbIMPDH upregulation for IMP pool expansion as TAS1R1/TAS1R3 synergist
• ▸Purine nucleoside phosphorylase AbPNP optimization via directed evolution for 2.3× Kcat improvement
• ▸Guanylate kinase AbGMPK pathway augmentation for GMP co-accumulation as secondary umami nucleotide
• ▸RNA degradation pathway redirection via AbRNase knockdown for free 5-IMP and 5-GMP accumulation from endogenous RNA pools
• ▸AbGS promoter engineering with synthetic UAS elements for constitutive high-expression glutamine synthetase output
• ▸AbGOGAT isoform 2 introduced for NADH-dependent glutamate synthesis as aerobic complement
• ▸AbVGT3 vacuolar glutamate transporter heterologous expression from Pleurotus ostreatus for expanded storage
• ▸AbAMPDA AMP deaminase downregulation to preserve adenylate pool for nucleotide co-agonist balance
• ▸AbNAGK N-acetylglutamate kinase feedback-resistant mutant for arginine-to-glutamate overflow routing
• ▸AbGDH isoform switch to mitochondrial targeting sequence variant for TCA-coupled glutamate burst during sporulation
• ▸AbGlu1 glutamate exporter upregulation for enhanced apoplastic free glutamate in dried state

Key Interventions