MSG Equivalent II-PR-A

Agaricus bisporus

Mushroom

Tier IIMSG Equivalent

Target Trait

4.25×

fold-change

MSG-equivalent umami intensity

Technical Specifications

Genomic Edits

GC Content

43-49%

Construct Length

2,160 bp

Promoter System

Ubiquitin / T7

Expression System

Protoplast PEG-fusion

Host Strain

PEG-mediated

Receptor Target

TAS1R1/TAS1R3 + mGluR1

IFI Score

361.3

Pathway Edits

AbGDH overexpression under constitutive Agaricus gpd promoter for alpha-ketoglutarate to glutamate maximal flux
Inosine 5-monophosphate synthase AbIMPDH upregulation for IMP pool expansion as TAS1R1/TAS1R3 synergist
Purine nucleoside phosphorylase AbPNP optimization via directed evolution for 2.3× Kcat improvement
Guanylate kinase AbGMPK pathway augmentation for GMP co-accumulation as secondary umami nucleotide
RNA degradation pathway redirection via AbRNase knockdown for free 5-IMP and 5-GMP accumulation from endogenous RNA pools
AbGS promoter engineering with synthetic UAS elements for constitutive high-expression glutamine synthetase output
AbGOGAT isoform 2 introduced for NADH-dependent glutamate synthesis as aerobic complement

Key Interventions

Synergistic glutamate + 5-IMP + 5-GMP triple co-accumulation for maximum TAS1R1/TAS1R3 receptor activationTAS1R1/TAS1R3 dual ligand optimization achieving umami intensity equivalent to exogenous MSG additionCell lysis-triggered flavor release via compartmentalized glutamate and nucleotide pools

IFI Subscale Profile

IFI Profile

IFI-S

—

IFI-U

361.3

IFI-B

—

IFI-K

—

IFI-C

—

IFI-P

—

IFI-A

—

IFI-F

—

Use Case

Dried mushroom powder, umami concentrates, and stock base applications replacing synthetic MSG. Certified-clean-label solution for food manufacturers seeking natural umami equivalence at scale.

License

$99 · Tier II License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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