Specimen SS-F-UMA-090 // UMAMI · IFI-U
MSG Equivalent II-AG-B
Agaricus bisporus
Mushroom
Target Trait
4.89×
fold-change
MSG-equivalent umami intensity
Technical Specifications
Genomic Edits
8
GC Content
43-49%
Construct Length
2,240 bp
Promoter System
Tissue-specific 2A11
Expression System
Agrobacterium-mediated T-DNA
Host Strain
EHA105
Receptor Target
TAS1R1/TAS1R3 + mGluR1
IFI Score
415.6
Pathway Edits
- AbGDH overexpression under constitutive Agaricus gpd promoter for alpha-ketoglutarate to glutamate maximal flux
- Inosine 5-monophosphate synthase AbIMPDH upregulation for IMP pool expansion as TAS1R1/TAS1R3 synergist
- Purine nucleoside phosphorylase AbPNP optimization via directed evolution for 2.3× Kcat improvement
- Guanylate kinase AbGMPK pathway augmentation for GMP co-accumulation as secondary umami nucleotide
- RNA degradation pathway redirection via AbRNase knockdown for free 5-IMP and 5-GMP accumulation from endogenous RNA pools
- AbGS promoter engineering with synthetic UAS elements for constitutive high-expression glutamine synthetase output
- AbGOGAT isoform 2 introduced for NADH-dependent glutamate synthesis as aerobic complement
- AbVGT3 vacuolar glutamate transporter heterologous expression from Pleurotus ostreatus for expanded storage
Key Interventions
IFI Subscale Profile
IFI Profile
Use Case
Dried mushroom powder, umami concentrates, and stock base applications replacing synthetic MSG. Certified-clean-label solution for food manufacturers seeking natural umami equivalence at scale.
License
$149 · Tier II License
Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.
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