Specimen SS-F-UMA-088 // UMAMI · IFI-U
MSG Equivalent I-IV-B
Agaricus bisporus
Mushroom
Target Trait
3.45×
fold-change
MSG-equivalent umami intensity
Technical Specifications
Genomic Edits
6
GC Content
42-48%
Construct Length
1,680 bp
Promoter System
T7/SP6 cell-free
Expression System
In-vitro cell-free
Host Strain
Cell-free
Receptor Target
TAS1R1/TAS1R3 + mGluR1
IFI Score
293.3
Pathway Edits
- AbGDH overexpression under constitutive Agaricus gpd promoter for alpha-ketoglutarate to glutamate maximal flux
- Inosine 5-monophosphate synthase AbIMPDH upregulation for IMP pool expansion as TAS1R1/TAS1R3 synergist
- Purine nucleoside phosphorylase AbPNP optimization via directed evolution for 2.3× Kcat improvement
- Guanylate kinase AbGMPK pathway augmentation for GMP co-accumulation as secondary umami nucleotide
- RNA degradation pathway redirection via AbRNase knockdown for free 5-IMP and 5-GMP accumulation from endogenous RNA pools
- AbGS promoter engineering with synthetic UAS elements for constitutive high-expression glutamine synthetase output
Key Interventions
IFI Subscale Profile
IFI Profile
Use Case
Dried mushroom powder, umami concentrates, and stock base applications replacing synthetic MSG. Certified-clean-label solution for food manufacturers seeking natural umami equivalence at scale.
License
$99 · Tier I License
Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.
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