IFI-U · MSG Equivalent

Specimen SS-F-UMA-085 // UMAMI · IFI-U

MSG Equivalent I-PR-A

Agaricus bisporus

Mushroom

Tier IMSG Equivalent

Target Trait

3×

fold-change

MSG-equivalent umami intensity

Technical Specifications

Genomic Edits

5

GC Content

42-48%

Construct Length

1,600 bp

Promoter System

Ubiquitin / T7

Expression System

Protoplast PEG-fusion

Host Strain

PEG-mediated

Receptor Target

TAS1R1/TAS1R3 + mGluR1

IFI Score

255

Pathway Edits

  • AbGDH overexpression under constitutive Agaricus gpd promoter for alpha-ketoglutarate to glutamate maximal flux
  • Inosine 5-monophosphate synthase AbIMPDH upregulation for IMP pool expansion as TAS1R1/TAS1R3 synergist
  • Purine nucleoside phosphorylase AbPNP optimization via directed evolution for 2.3× Kcat improvement
  • Guanylate kinase AbGMPK pathway augmentation for GMP co-accumulation as secondary umami nucleotide
  • RNA degradation pathway redirection via AbRNase knockdown for free 5-IMP and 5-GMP accumulation from endogenous RNA pools

Key Interventions

Synergistic glutamate + 5-IMP + 5-GMP triple co-accumulation for maximum TAS1R1/TAS1R3 receptor activationTAS1R1/TAS1R3 dual ligand optimization achieving umami intensity equivalent to exogenous MSG additionCell lysis-triggered flavor release via compartmentalized glutamate and nucleotide pools

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
255
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

Dried mushroom powder, umami concentrates, and stock base applications replacing synthetic MSG. Certified-clean-label solution for food manufacturers seeking natural umami equivalence at scale.

License

$49 · Tier I License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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