Specimen SS-F-UMA-075 // UMAMI · IFI-U
Glutamate Peak V-BL-A
Capsicum chinense
Pepper
Target Trait
6×
fold-change
Free glutamate peak concentration
Technical Specifications
Genomic Edits
10
GC Content
46-52%
Construct Length
3,600 bp
Promoter System
CaMV 35S / Ubiquitin
Expression System
Biolistic transformation
Host Strain
Particle Gun
Receptor Target
TAS1R1/TAS1R3 + mGluR1
IFI Score
510
Pathway Edits
- CcGS1 overexpression under pericarp-specific Cc2A11 promoter for compartment-targeted glutamine synthetase boost
- CcGDH2 allosteric tuning via N-terminal domain truncation for higher Vmax without ADP inhibition
- GABA shunt suppression via CcSSADH partial RNAi knockdown to redirect succinate-semialdehyde back to glutamate
- Vacuolar H+-ATPase CcVHA-A subunit upregulation for enhanced glutamate compartmentalization driving cytosolic replenishment
- Feedback-resistant CcGLN1 mutant (G297A, R299K) for glutamine synthetase desensitization to glutamine product inhibition
- CcGDH1 isoform 2 heterologous expression from Arabidopsis for expanded deamination capacity under low-carbon conditions
- CcAAT2 aminotransferase overexpression for enhanced oxaloacetate-to-aspartate-to-glutamate carbon routing
- CcGO1 glutamate oxidase knockdown to prevent futile cycling of free glutamate pool
- CcPEPC1 phosphoenolpyruvate carboxylase upregulation for increased OAA supply to TCA-glutamate interface
- CcSucSy1 sucrose synthase downregulation to redirect carbon flux toward amino acid biosynthesis
Key Interventions
IFI Subscale Profile
IFI Profile
Use Case
Pepper mash, hot sauce fermentation substrates, and extract standardization for food manufacturing. Designed for fermented capsicum products where free glutamate amplifies fermentation-derived savory complexity.
License
$499 · Tier V License
Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.
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