IFI-U · Glutamate Peak

Specimen SS-F-UMA-053 // UMAMI · IFI-U

Glutamate Peak II-PR-A

Capsicum chinense

Pepper

Tier IIGlutamate Peak

Target Trait

3×

fold-change

Free glutamate peak concentration

Technical Specifications

Genomic Edits

6

GC Content

43-49%

Construct Length

2,080 bp

Promoter System

Ubiquitin / T7

Expression System

Protoplast PEG-fusion

Host Strain

PEG-mediated

Receptor Target

TAS1R1/TAS1R3 + mGluR1

IFI Score

255

Pathway Edits

  • CcGS1 overexpression under pericarp-specific Cc2A11 promoter for compartment-targeted glutamine synthetase boost
  • CcGDH2 allosteric tuning via N-terminal domain truncation for higher Vmax without ADP inhibition
  • GABA shunt suppression via CcSSADH partial RNAi knockdown to redirect succinate-semialdehyde back to glutamate
  • Vacuolar H+-ATPase CcVHA-A subunit upregulation for enhanced glutamate compartmentalization driving cytosolic replenishment
  • Feedback-resistant CcGLN1 mutant (G297A, R299K) for glutamine synthetase desensitization to glutamine product inhibition
  • CcGDH1 isoform 2 heterologous expression from Arabidopsis for expanded deamination capacity under low-carbon conditions

Key Interventions

Free amino acid pool redirection from GABA and alanine pathways toward net glutamate accumulationTAS1R1/TAS1R3 co-agonist enhancement via IMP co-accumulation through purine salvage pathway modulationSynergistic IMP/GMP co-enrichment via nucleotide transporter upregulation

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
255
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

Pepper mash, hot sauce fermentation substrates, and extract standardization for food manufacturing. Designed for fermented capsicum products where free glutamate amplifies fermentation-derived savory complexity.

License

$99 · Tier II License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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