IFI-U · Glutamate Peak

Specimen SS-F-UMA-048 // UMAMI · IFI-U

Glutamate Peak I-IV-B

Capsicum chinense

Pepper

Tier IGlutamate Peak

Target Trait

2.3×

fold-change

Free glutamate peak concentration

Technical Specifications

Genomic Edits

5

GC Content

42-48%

Construct Length

1,600 bp

Promoter System

T7/SP6 cell-free

Expression System

In-vitro cell-free

Host Strain

Cell-free

Receptor Target

TAS1R1/TAS1R3 + mGluR1

IFI Score

195.5

Pathway Edits

  • CcGS1 overexpression under pericarp-specific Cc2A11 promoter for compartment-targeted glutamine synthetase boost
  • CcGDH2 allosteric tuning via N-terminal domain truncation for higher Vmax without ADP inhibition
  • GABA shunt suppression via CcSSADH partial RNAi knockdown to redirect succinate-semialdehyde back to glutamate
  • Vacuolar H+-ATPase CcVHA-A subunit upregulation for enhanced glutamate compartmentalization driving cytosolic replenishment
  • Feedback-resistant CcGLN1 mutant (G297A, R299K) for glutamine synthetase desensitization to glutamine product inhibition

Key Interventions

Free amino acid pool redirection from GABA and alanine pathways toward net glutamate accumulationTAS1R1/TAS1R3 co-agonist enhancement via IMP co-accumulation through purine salvage pathway modulationSynergistic IMP/GMP co-enrichment via nucleotide transporter upregulation

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
195.5
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

Pepper mash, hot sauce fermentation substrates, and extract standardization for food manufacturing. Designed for fermented capsicum products where free glutamate amplifies fermentation-derived savory complexity.

License

$99 · Tier I License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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