IFI-U · Savory Flux
Specimen SS-F-UMA-035 // UMAMI · IFI-U
Savory Flux V-BL-A
Solanum lycopersicum
Tomato
Tier VSavory Flux
Target Trait
4×
fold-change
Glutamate flux amplification
Technical Specifications
Genomic Edits
8
GC Content
46-52%
Construct Length
3,440 bp
Promoter System
CaMV 35S / Ubiquitin
Expression System
Biolistic transformation
Host Strain
Particle Gun
Receptor Target
TAS1R1/TAS1R3 + mGluR1
IFI Score
340
Pathway Edits
- SlGDH1 promoter swap to CaMV35S for constitutive glutamate dehydrogenase expression
- SlGOGAT upregulation via synthetic fruit-ripening promoter for enhanced glutamine:2-oxoglutarate aminotransferase activity
- Vacuolar glutamate transporter SlVGT1 overexpression for apoplastic glutamate accumulation
- SlGS2 activity boost via T-DNA insertion at native locus for plastidic glutamine synthetase amplification
- SlAAT1 aspartate aminotransferase redirected to favor glutamate over aspartate production
- Feedback-resistant SlGS1 cytosolic isoform introduced for unregulated glutamate synthesis
- SlGln1;2 pericarp-specific overexpression for localized free amino acid enrichment
- SlNADH-GOGAT secondary isoform introduced under ER-stress promoter for overflow capacity
Key Interventions
TAS1R1/TAS1R3 binding-site glutamate pool expansion via GDH/GS dual upregulationGlutamate vacuolar compartmentalization for controlled release during tissue disruptionmGluR1 sensitization via reduced competing amino acid background
IFI Subscale Profile
IFI Profile
IFI-S—
IFI-U340
IFI-B—
IFI-K—
IFI-C—
IFI-P—
IFI-A—
IFI-F—
Use Case
Fresh tomato and processed sauce products requiring enhanced savory base note depth. Ideal for ketchup, tomato paste, pizza sauce, and whole-fruit fresh-market applications.
License
$499 · Tier V License
Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.
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