IFI-U · Savory Flux

Specimen SS-F-UMA-025 // UMAMI · IFI-U

Savory Flux IV-AG-A

Solanum lycopersicum

Tomato

Tier IVSavory Flux

Target Trait

3.38×

fold-change

Glutamate flux amplification

Technical Specifications

Genomic Edits

7

GC Content

45-51%

Construct Length

2,960 bp

Promoter System

Tissue-specific 2A11

Expression System

Agrobacterium-mediated T-DNA

Host Strain

EHA105

Receptor Target

TAS1R1/TAS1R3 + mGluR1

IFI Score

287.3

Pathway Edits

  • SlGDH1 promoter swap to CaMV35S for constitutive glutamate dehydrogenase expression
  • SlGOGAT upregulation via synthetic fruit-ripening promoter for enhanced glutamine:2-oxoglutarate aminotransferase activity
  • Vacuolar glutamate transporter SlVGT1 overexpression for apoplastic glutamate accumulation
  • SlGS2 activity boost via T-DNA insertion at native locus for plastidic glutamine synthetase amplification
  • SlAAT1 aspartate aminotransferase redirected to favor glutamate over aspartate production
  • Feedback-resistant SlGS1 cytosolic isoform introduced for unregulated glutamate synthesis
  • SlGln1;2 pericarp-specific overexpression for localized free amino acid enrichment

Key Interventions

TAS1R1/TAS1R3 binding-site glutamate pool expansion via GDH/GS dual upregulationGlutamate vacuolar compartmentalization for controlled release during tissue disruptionmGluR1 sensitization via reduced competing amino acid background

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
287.3
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

Fresh tomato and processed sauce products requiring enhanced savory base note depth. Ideal for ketchup, tomato paste, pizza sauce, and whole-fruit fresh-market applications.

License

$249 · Tier IV License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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