IFI-U · Savory Flux

Specimen SS-F-UMA-013 // UMAMI · IFI-U

Savory Flux II-PR-A

Solanum lycopersicum

Tomato

Tier IISavory Flux

Target Trait

2.13×

fold-change

Glutamate flux amplification

Technical Specifications

Genomic Edits

4

GC Content

43-49%

Construct Length

1,920 bp

Promoter System

Ubiquitin / T7

Expression System

Protoplast PEG-fusion

Host Strain

PEG-mediated

Receptor Target

TAS1R1/TAS1R3 + mGluR1

IFI Score

181

Pathway Edits

  • SlGDH1 promoter swap to CaMV35S for constitutive glutamate dehydrogenase expression
  • SlGOGAT upregulation via synthetic fruit-ripening promoter for enhanced glutamine:2-oxoglutarate aminotransferase activity
  • Vacuolar glutamate transporter SlVGT1 overexpression for apoplastic glutamate accumulation
  • SlGS2 activity boost via T-DNA insertion at native locus for plastidic glutamine synthetase amplification

Key Interventions

TAS1R1/TAS1R3 binding-site glutamate pool expansion via GDH/GS dual upregulationGlutamate vacuolar compartmentalization for controlled release during tissue disruptionmGluR1 sensitization via reduced competing amino acid background

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
181
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

Fresh tomato and processed sauce products requiring enhanced savory base note depth. Ideal for ketchup, tomato paste, pizza sauce, and whole-fruit fresh-market applications.

License

$99 · Tier II License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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