IFI-P · Wasabi Tier

Specimen SS-F-TRP-200 // PUNGENT · IFI-P

Wasabi Tier V-IV-B

Sinapis alba

White Mustard

Tier VWasabi Tier

Target Trait

9.2×

fold-change

Wasabi-analog pungency fold-change

Technical Specifications

Genomic Edits

15

GC Content

44.2%

Construct Length

7,055 bp

Promoter System

T7/SP6 cell-free

Expression System

In-vitro cell-free

Host Strain

Cell-free

Receptor Target

TRPA1

IFI Score

1,150

Pathway Edits

  • WasS1 glucosinolate synthase analog integration from Wasabia japonica functional ortholog
  • TGG2 high-volatility myrosinase isoform with pH 5.5-optimized kinetic profile
  • PEN2 myrosinase pathway integration for stomatal-independent rapid hydrolysis
  • MYB34 aliphatic GSL transcription factor with ABA-independent constitutive promoter cassette
  • CYP79A1 + CYP79B2 dual aromatic-aliphatic branch upregulation for layered ITC profile
  • FMOGS-OX1 flux redirection from methylsulfinyl to alkenyl glucosinolate branch
  • AOP2 isothiocyanate-forming enzyme optimization for AITC chain-length specificity
  • UGT74C1 glucosyltransferase wasabi-isoform analog for 6-methylsulfinylhexyl ITC synthesis
  • Volatile AITC release kinetics engineering via apoplastic cell-wall permeabilization
  • Shelf-stable glucosinolate precursor accumulation via vacuolar partitioning enhancement
  • MAM1 methionine chain elongation with expanded alkenyl substrate specificity range
  • CYP83A1 high-flux aliphatic pathway integration for sinigrin-to-AITC complete cascade
  • TGG1 + TGG3 myrosinase complement for exhaustive cell-type-specific hydrolysis coverage
  • AtST5c sulfotransferase final-step optimization for complete desulfo-glucosinolate processing
  • WasR1-analog TRPA1-response regulatory element for pungency-feedback circuit integration

Key Interventions

Wasabi-analog volatile AITC maximization via WasS1 ortholog and dual-CYP79 pathway engineeringPEN2-mediated stomatal-independent hydrolysis enabling rapid sensory pungency onset kineticsShelf-stable glucosinolate precursor design for consistent supply-chain pungency performanceVolatile AITC release kinetics optimized for nasal trigeminal activation profile matching Wasabia japonicaCell-free T7/SP6 translation system for in-vitro pungency pathway enzyme validation

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
1,150
IFI-A
—
IFI-F
—

Use Case

Tier V wasabi-substitute ingredient system for premium sushi applications, Japanese cuisine condiment formulation, and authentic-profile wasabi paste manufacturing where Wasabia japonica supply constraints or agronomic cost considerations require an engineered botanical equivalent. Suitable for RTD condiment manufacturers, fine-dining ingredient suppliers, and specialty food brands targeting the wasabi premium segment with verifiable pungency specifications.

License

$549 · Tier V License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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