IFI-P · TRPA1 Scale

Specimen SS-F-TRP-155 // PUNGENT · IFI-P

TRPA1 Scale V-BL-A

Brassica nigra

Black Mustard

Tier VTRPA1 Scale

Target Trait

7×

fold-change

TRPA1 activation index fold-change

Technical Specifications

Genomic Edits

12

GC Content

48.7%

Construct Length

5,950 bp

Promoter System

CaMV 35S / Ubiquitin

Expression System

Biolistic transformation

Host Strain

Particle Gun

Receptor Target

TRPA1

IFI Score

875

Pathway Edits

  • MYB28 + MYB29 co-overexpression via T2A self-cleaving peptide bicistronic construct
  • CYP79A2 + CYP79F1 dual upregulation for expanded aliphatic glucosinolate chain range
  • TGG1 + TGG2 myrosinase isoform stacking for complete substrate pH coverage
  • MAM1 and MAM3 methionine chain elongation enzyme upregulation for C3-C8 GSL range
  • AtST5a + AtST5b sulfotransferase dual integration for high-throughput desulfo conversion
  • BGLU28 beta-glucosidase optimization for broad glucosinolate substrate mobilization
  • AOP2 isothiocyanate-forming enzyme overexpression for ITC pathway dominance
  • CYP83A1 + CYP83B1 dual-branch pathway flux amplification
  • TGG4 apoplastic myrosinase integration for extracellular AITC burst-release
  • GSH glutathione biosynthesis pathway enhancement for glucosinolate cofactor supply
  • MBP-domain glucosinolate binding protein disruption for vacuolar GSL mobilization
  • Vacuolar GLT1 transporter capacity expansion for maximal GSL storage and release

Key Interventions

Multi-glucosinolate spectrum broadening via MYB28/MYB29 T2A co-overexpressionMyrosinase isoform stacking for complete glucosinolate hydrolysis across pH 4.5-7.5Sulfotransferase pathway optimization enabling high-throughput desulfo-glucosinolate processingTRPA1 activation index confirmed by validated calcium-flux assay with IFI-P panel scoringBiolistic bombardment parameters optimized for high-copy tandem stable integration events

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
875
IFI-A
—
IFI-F
—

Use Case

Tier V high-potency TRPA1 activator platform for premium specialty mustard production, wasabi-grade flavor applications, and research-grade TRPA1 agonist compound production. Suitable for functional food development requiring precisely defined pungency thresholds, and for flavor house R&D programs targeting novel heat modalities beyond conventional capsaicin-centric Scoville frameworks.

License

$499 · Tier V License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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