Specimen SS-F-TRP-152 // PUNGENT · IFI-P
TRPA1 Scale IV-IV-B
Brassica nigra
Black Mustard
Target Trait
7.19×
fold-change
TRPA1 activation index fold-change
Technical Specifications
Genomic Edits
12
GC Content
45.9%
Construct Length
5,868 bp
Promoter System
T7/SP6 cell-free
Expression System
In-vitro cell-free
Host Strain
Cell-free
Receptor Target
TRPA1
IFI Score
898.8
Pathway Edits
- MYB28 + MYB29 co-overexpression via T2A self-cleaving peptide bicistronic construct
- CYP79A2 + CYP79F1 dual upregulation for expanded aliphatic glucosinolate chain range
- TGG1 + TGG2 myrosinase isoform stacking for complete substrate pH coverage
- MAM1 and MAM3 methionine chain elongation enzyme upregulation for C3-C8 GSL range
- AtST5a + AtST5b sulfotransferase dual integration for high-throughput desulfo conversion
- BGLU28 beta-glucosidase optimization for broad glucosinolate substrate mobilization
- AOP2 isothiocyanate-forming enzyme overexpression for ITC pathway dominance
- CYP83A1 + CYP83B1 dual-branch pathway flux amplification
- TGG4 apoplastic myrosinase integration for extracellular AITC burst-release
- GSH glutathione biosynthesis pathway enhancement for glucosinolate cofactor supply
- MBP-domain glucosinolate binding protein disruption for vacuolar GSL mobilization
- Vacuolar GLT1 transporter capacity expansion for maximal GSL storage and release
Key Interventions
IFI Subscale Profile
IFI Profile
Use Case
Tier IV high-potency TRPA1 activator platform for premium specialty mustard production, wasabi-grade flavor applications, and research-grade TRPA1 agonist compound production. Suitable for functional food development requiring precisely defined pungency thresholds, and for flavor house R&D programs targeting novel heat modalities beyond conventional capsaicin-centric Scoville frameworks.
License
$299 · Tier IV License
Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.
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