Specimen SS-F-TRP-114 // PUNGENT · IFI-P
Pungent Peak V-AG-B
Capsicum annuum
Pepper
Target Trait
6.9×
fold-change
TRPA1 agonist fold-change
Technical Specifications
Genomic Edits
12
GC Content
43.6%
Construct Length
5,853 bp
Promoter System
Tissue-specific 2A11
Expression System
Agrobacterium-mediated T-DNA
Host Strain
EHA105
Receptor Target
TRPA1
IFI Score
862.5
Pathway Edits
- Heterologous MYB28 codon-optimized integration in Capsicum annuum genome
- CYP79A2 heterologous expression for allyl glucosinolate oxime pathway establishment
- TGG2 high-activity myrosinase isoform insertion for efficient AITC release
- CYP83A1 upregulation for S-alkyl-thiohydroximate lyase activity in Capsicum
- SUR1-encoding construct integration for isothiocyanate selectivity in heterologous host
- UGT74B1 hetero-glucosyltransferase adapted for Capsicum metabolic background
- Capsaicin synthase CS2 pathway modulation for AITC-to-capsaicin ratio control
- DREB2-like cold-stress promoter integration for volatile AITC burst-release
- OMT3 O-methyltransferase for capsaicin-ITC co-production ratio fine-tuning
- pAMT downregulation for AITC/capsaicin balance shift toward TRPA1 agonist dominance
- Vacuolar targeting signal engineering for glucosinolate precursor compartmentalization
- Regulatory network insulation elements for heterologous GSL pathway transcriptional stability
Key Interventions
IFI Subscale Profile
IFI Profile
Use Case
Tier V dual-pungency platform for complex layered heat profiles in hot sauces, artisanal chili blends, and specialty condiments requiring TRPA1-mediated mustard-heat onset paired with TRPV1-mediated capsaicin warmth. Ideal for product developers seeking novel pungency signatures not achievable through conventional botanical blending or capsaicin extraction alone.
License
$549 · Tier V License
Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.
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