IFI-P · Pungent Peak

Specimen SS-F-TRP-108 // PUNGENT · IFI-P

Pungent Peak IV-BL-B

Capsicum annuum

Pepper

Tier IVPungent Peak

Target Trait

6.04×

fold-change

TRPA1 agonist fold-change

Technical Specifications

Genomic Edits

11

GC Content

43.4%

Construct Length

5,540 bp

Promoter System

CaMV 35S / Ubiquitin

Expression System

Biolistic transformation

Host Strain

Particle Gun

Receptor Target

TRPA1

IFI Score

755

Pathway Edits

  • Heterologous MYB28 codon-optimized integration in Capsicum annuum genome
  • CYP79A2 heterologous expression for allyl glucosinolate oxime pathway establishment
  • TGG2 high-activity myrosinase isoform insertion for efficient AITC release
  • CYP83A1 upregulation for S-alkyl-thiohydroximate lyase activity in Capsicum
  • SUR1-encoding construct integration for isothiocyanate selectivity in heterologous host
  • UGT74B1 hetero-glucosyltransferase adapted for Capsicum metabolic background
  • Capsaicin synthase CS2 pathway modulation for AITC-to-capsaicin ratio control
  • DREB2-like cold-stress promoter integration for volatile AITC burst-release
  • OMT3 O-methyltransferase for capsaicin-ITC co-production ratio fine-tuning
  • pAMT downregulation for AITC/capsaicin balance shift toward TRPA1 agonist dominance
  • Vacuolar targeting signal engineering for glucosinolate precursor compartmentalization

Key Interventions

Complete heterologous glucosinolate biosynthesis pathway established in Capsicum annuumAITC-capsaicin synergy engineering for dual TRPA1/TRPV1 agonist co-productionPungency onset and duration tuning through metabolic compartmentalization designBiolistic bombardment parameters optimized for high-copy tandem stable integration events

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
755
IFI-A
—
IFI-F
—

Use Case

Tier IV dual-pungency platform for complex layered heat profiles in hot sauces, artisanal chili blends, and specialty condiments requiring TRPA1-mediated mustard-heat onset paired with TRPV1-mediated capsaicin warmth. Ideal for product developers seeking novel pungency signatures not achievable through conventional botanical blending or capsaicin extraction alone.

License

$299 · Tier IV License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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