Pungent Peak I-IV-A

Capsicum annuum

Pepper

Tier IPungent Peak

Target Trait

3×

fold-change

TRPA1 agonist fold-change

Technical Specifications

Genomic Edits

GC Content

46%

Construct Length

3,210 bp

Promoter System

T7/SP6 cell-free

Expression System

In-vitro cell-free

Host Strain

Cell-free

Receptor Target

TRPA1

IFI Score

375

Pathway Edits

Heterologous MYB28 codon-optimized integration in Capsicum annuum genome
CYP79A2 heterologous expression for allyl glucosinolate oxime pathway establishment
TGG2 high-activity myrosinase isoform insertion for efficient AITC release
CYP83A1 upregulation for S-alkyl-thiohydroximate lyase activity in Capsicum
SUR1-encoding construct integration for isothiocyanate selectivity in heterologous host

Key Interventions

Complete heterologous glucosinolate biosynthesis pathway established in Capsicum annuumAITC-capsaicin synergy engineering for dual TRPA1/TRPV1 agonist co-productionPungency onset and duration tuning through metabolic compartmentalization designCell-free T7/SP6 translation system for in-vitro pungency pathway enzyme validation

IFI Subscale Profile

IFI Profile

IFI-S

—

IFI-U

—

IFI-B

—

IFI-K

—

IFI-C

—

IFI-P

375

IFI-A

—

IFI-F

—

Use Case

Tier I dual-pungency platform for complex layered heat profiles in hot sauces, artisanal chili blends, and specialty condiments requiring TRPA1-mediated mustard-heat onset paired with TRPV1-mediated capsaicin warmth. Ideal for product developers seeking novel pungency signatures not achievable through conventional botanical blending or capsaicin extraction alone.

License

$49 · Tier I License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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