IFI-P · Pungent Peak

Specimen SS-F-TRP-086 // PUNGENT · IFI-P

Pungent Peak I-PR-B

Capsicum annuum

Pepper

Tier IPungent Peak

Target Trait

3.45×

fold-change

TRPA1 agonist fold-change

Technical Specifications

Genomic Edits

6

GC Content

44.7%

Construct Length

3,573 bp

Promoter System

Ubiquitin / T7

Expression System

Protoplast PEG-fusion

Host Strain

PEG-mediated

Receptor Target

TRPA1

IFI Score

431.3

Pathway Edits

  • Heterologous MYB28 codon-optimized integration in Capsicum annuum genome
  • CYP79A2 heterologous expression for allyl glucosinolate oxime pathway establishment
  • TGG2 high-activity myrosinase isoform insertion for efficient AITC release
  • CYP83A1 upregulation for S-alkyl-thiohydroximate lyase activity in Capsicum
  • SUR1-encoding construct integration for isothiocyanate selectivity in heterologous host
  • UGT74B1 hetero-glucosyltransferase adapted for Capsicum metabolic background

Key Interventions

Complete heterologous glucosinolate biosynthesis pathway established in Capsicum annuumAITC-capsaicin synergy engineering for dual TRPA1/TRPV1 agonist co-productionPungency onset and duration tuning through metabolic compartmentalization designPEG-mediated transient expression protocol validated for rapid phenotypic pathway screening

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
431.3
IFI-A
—
IFI-F
—

Use Case

Tier I dual-pungency platform for complex layered heat profiles in hot sauces, artisanal chili blends, and specialty condiments requiring TRPA1-mediated mustard-heat onset paired with TRPV1-mediated capsaicin warmth. Ideal for product developers seeking novel pungency signatures not achievable through conventional botanical blending or capsaicin extraction alone.

License

$99 · Tier I License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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