Specimen SS-F-TRP-074 // PUNGENT · IFI-P
Isothiocyanate Grade V-AG-B
Sinapis alba
White Mustard
Target Trait
5.17×
fold-change
Isothiocyanate yield fold-change
Technical Specifications
Genomic Edits
10
GC Content
43.6%
Construct Length
5,013 bp
Promoter System
Tissue-specific 2A11
Expression System
Agrobacterium-mediated T-DNA
Host Strain
EHA105
Receptor Target
TRPA1
IFI Score
646.3
Pathway Edits
- CYP79A1 upregulation for aromatic glucosinolate oxime biosynthesis
- GSL-OH glucosinolate hydroxylase suppression for ITC pathway dominance
- MYR1 myrosinase enhancement (3x baseline catalytic activity)
- ESP-like epithiospecifier modifier knockdown for ITC selectivity over nitrile
- AtST5a sulfotransferase pathway flux increase for desulfoglucosinolate conversion
- CYP83B1 N-hydroxy-phenylacetaldoxime dehydratase upregulation
- SUR1 C-S lyase integration for broad-substrate glucosinolate processing
- BGLU28 beta-glucosidase co-expression for glucosinolate mobilization
- AOP3-null allele introduction to redirect flux toward ITC-forming AOP2 branch
- Vacuolar GLT1 transporter engineering for enhanced glucosinolate storage and burst-release kinetics
Key Interventions
IFI Subscale Profile
IFI Profile
Use Case
Tier V isothiocyanate-enriched ingredient platform for cruciferous flavor enhancement, horseradish-analog development, and TRPA1-selective pungency in dressings, sauces, and marinades. Designed for food manufacturers seeking consistent, high-yield ITC content without the enzymatic variability or supply volatility inherent in conventional botanical White Mustard sources.
License
$549 · Tier V License
Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.
Already purchased?