Isothiocyanate Grade III-PR-A

Sinapis alba

White Mustard

Tier IIIIsothiocyanate Grade

Target Trait

3.25×

fold-change

Isothiocyanate yield fold-change

Technical Specifications

Genomic Edits

GC Content

47.6%

Construct Length

3,990 bp

Promoter System

Ubiquitin / T7

Expression System

Protoplast PEG-fusion

Host Strain

PEG-mediated

Receptor Target

TRPA1

IFI Score

406.3

Pathway Edits

CYP79A1 upregulation for aromatic glucosinolate oxime biosynthesis
GSL-OH glucosinolate hydroxylase suppression for ITC pathway dominance
MYR1 myrosinase enhancement (3x baseline catalytic activity)
ESP-like epithiospecifier modifier knockdown for ITC selectivity over nitrile
AtST5a sulfotransferase pathway flux increase for desulfoglucosinolate conversion
CYP83B1 N-hydroxy-phenylacetaldoxime dehydratase upregulation
SUR1 C-S lyase integration for broad-substrate glucosinolate processing

Key Interventions

Sinalbin-to-benzyl ITC pathway redirect with maximal TRPA1 receptor specificityIsothiocyanate yield maximization through ESP suppression and MYR1 upregulationCell-wall glucosinolate localization engineering for pungency release on demandPEG-mediated transient expression protocol validated for rapid phenotypic pathway screening

IFI Subscale Profile

IFI Profile

IFI-S

—

IFI-U

—

IFI-B

—

IFI-K

—

IFI-C

—

IFI-P

406.3

IFI-A

—

IFI-F

—

Use Case

Tier III isothiocyanate-enriched ingredient platform for cruciferous flavor enhancement, horseradish-analog development, and TRPA1-selective pungency in dressings, sauces, and marinades. Designed for food manufacturers seeking consistent, high-yield ITC content without the enzymatic variability or supply volatility inherent in conventional botanical White Mustard sources.

License

$149 · Tier III License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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