IFI-P · Isothiocyanate Grade

Specimen SS-F-TRP-048 // PUNGENT · IFI-P

Isothiocyanate Grade I-IV-B

Sinapis alba

White Mustard

Tier IIsothiocyanate Grade

Target Trait

2.3×

fold-change

Isothiocyanate yield fold-change

Technical Specifications

Genomic Edits

5

GC Content

45.2%

Construct Length

3,147 bp

Promoter System

T7/SP6 cell-free

Expression System

In-vitro cell-free

Host Strain

Cell-free

Receptor Target

TRPA1

IFI Score

287.5

Pathway Edits

  • CYP79A1 upregulation for aromatic glucosinolate oxime biosynthesis
  • GSL-OH glucosinolate hydroxylase suppression for ITC pathway dominance
  • MYR1 myrosinase enhancement (3x baseline catalytic activity)
  • ESP-like epithiospecifier modifier knockdown for ITC selectivity over nitrile
  • AtST5a sulfotransferase pathway flux increase for desulfoglucosinolate conversion

Key Interventions

Sinalbin-to-benzyl ITC pathway redirect with maximal TRPA1 receptor specificityIsothiocyanate yield maximization through ESP suppression and MYR1 upregulationCell-wall glucosinolate localization engineering for pungency release on demandCell-free T7/SP6 translation system for in-vitro pungency pathway enzyme validation

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
287.5
IFI-A
—
IFI-F
—

Use Case

Tier I isothiocyanate-enriched ingredient platform for cruciferous flavor enhancement, horseradish-analog development, and TRPA1-selective pungency in dressings, sauces, and marinades. Designed for food manufacturers seeking consistent, high-yield ITC content without the enzymatic variability or supply volatility inherent in conventional botanical White Mustard sources.

License

$99 · Tier I License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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