IFI-P · Allyl Series

Specimen SS-F-TRP-040 // PUNGENT · IFI-P

Allyl Series V-IV-B

Brassica nigra

Black Mustard

Tier VAllyl Series

Target Trait

2.88×

fold-change

Allyl isothiocyanate equivalent fold-change

Technical Specifications

Genomic Edits

8

GC Content

44.2%

Construct Length

4,275 bp

Promoter System

T7/SP6 cell-free

Expression System

In-vitro cell-free

Host Strain

Cell-free

Receptor Target

TRPA1

IFI Score

360

Pathway Edits

  • MYB28 overexpression (2.5x transcriptional output increase)
  • CYP79A2 upregulation for chain-elongated aliphatic oxime biosynthesis
  • TGG1 myrosinase enhancement for complete sinigrin hydrolysis
  • CYP83A1 S-alkyl-thiohydroximate lyase flux optimization
  • SUR1 C-S lyase integration for allyl glucosinolate nitrile pathway suppression
  • UGT74B1 glucosyltransferase kinetics optimization for sinigrin assembly
  • STATa sulfotransferase aliphatic branch amplification
  • Upstream MYB28 promoter insulator element for feedback-resistance expression

Key Interventions

Sinigrin biosynthesis amplification via MYB28-driven transcriptional cascadeMyrosinase compartmentalization for controlled AITC release upon tissue disruptionAllyl glucosinolate-to-AITC conversion efficiency optimizationCell-free T7/SP6 translation system for in-vitro pungency pathway enzyme validation

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
360
IFI-A
—
IFI-F
—

Use Case

Tier V allyl-pungency enhancement for mustard condiment formulation, wasabi-style horseradish applications, and TRPA1-targeted flavor contrast in savory profiles. Suitable for controlled-release pungency in emulsified dressings, cold-process marinades, and charcuterie-adjacent products requiring sharp nasal pungency with a clean, non-lingering finish.

License

$549 · Tier V License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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