IFI-S · Crystal Grade

Specimen SS-F-SWT-200 // SWEET · IFI-S

Crystal Grade V-IV-B

Capsicum annuum

Pepper

Tier VCrystal Grade

Target Trait

9.2×

fold-change

Thaumatin-like sweet protein receptor potency fold-change

Technical Specifications

Genomic Edits

7

GC Content

46.7%

Construct Length

7,220 bp

Promoter System

T7/SP6 cell-free

Expression System

In-vitro cell-free

Host Strain

Cell-free

Receptor Target

TAS1R2/TAS1R3

IFI Score

920

Pathway Edits

  • Thaumatin II synthetic gene expression cassette with tissue-specific promoter
  • TAS1R2/TAS1R3 orthosteric sweet protein binding site expansion via VFT remodeling
  • TRPM5 conductance amplification via pore-loop S5-P helix engineering
  • Sweet taste receptor allosteric potentiator response element integration
  • Cyclic nucleotide phosphodiesterase 1C suppression cassette for sustained signaling
  • Taste cell-specific transcription factor Skn-1a overexpression for receptor density
  • Calcium-activated Cl- channel TMEM16A upregulation for signal amplification

Key Interventions

Thaumatin disulfide bridge architecture optimization preserving all 8 native disulfidesTAS1R2 CRD-TMD junction interface remodeling for macromolecular ligand accessTRPM5 N-terminal cytoplasmic calmodulin binding domain kinetics modificationReceptor heterodimer interface stabilization via engineered inter-subunit salt bridgesTaste bud progenitor cell differentiation enhancement via Skn-1a regulatory elementTMEM16A channel gating threshold lowering for amplified depolarization responseIP3 receptor clustering optimization at endoplasmic reticulum-plasma membrane junctions

IFI Subscale Profile

IFI Profile

IFI-S
920
IFI-U
—
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

Enhanced performance precision-fermented sweet protein production optimization platform with amplified TAS1R2/TAS1R3 receptor activation for premium-tier applications

License

$549 · Tier V License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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