IFI-S · Crystal Grade

Specimen SS-F-SWT-195 // SWEET · IFI-S

Crystal Grade V-BL-A

Capsicum annuum

Pepper

Tier VCrystal Grade

Target Trait

8×

fold-change

Thaumatin-like sweet protein receptor potency fold-change

Technical Specifications

Genomic Edits

6

GC Content

46.0%

Construct Length

7,060 bp

Promoter System

CaMV 35S / Ubiquitin

Expression System

Biolistic transformation

Host Strain

Particle Gun

Receptor Target

TAS1R2/TAS1R3

IFI Score

800

Pathway Edits

  • Thaumatin II synthetic gene expression cassette with tissue-specific promoter
  • TAS1R2/TAS1R3 orthosteric sweet protein binding site expansion via VFT remodeling
  • TRPM5 conductance amplification via pore-loop S5-P helix engineering
  • Sweet taste receptor allosteric potentiator response element integration
  • Cyclic nucleotide phosphodiesterase 1C suppression cassette for sustained signaling
  • Taste cell-specific transcription factor Skn-1a overexpression for receptor density

Key Interventions

Thaumatin disulfide bridge architecture optimization preserving all 8 native disulfidesTAS1R2 CRD-TMD junction interface remodeling for macromolecular ligand accessTRPM5 N-terminal cytoplasmic calmodulin binding domain kinetics modificationReceptor heterodimer interface stabilization via engineered inter-subunit salt bridgesTaste bud progenitor cell differentiation enhancement via Skn-1a regulatory elementTMEM16A channel gating threshold lowering for amplified depolarization response

IFI Subscale Profile

IFI Profile

IFI-S
800
IFI-U
—
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

precision-fermented sweet protein production optimization platform

License

$499 · Tier V License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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