IFI-S · Crystal Grade

Specimen SS-F-SWT-191 // SWEET · IFI-S

Crystal Grade IV-IV-A

Capsicum annuum

Pepper

Tier IVCrystal Grade

Target Trait

7.13×

fold-change

Thaumatin-like sweet protein receptor potency fold-change

Technical Specifications

Genomic Edits

5

GC Content

44.8%

Construct Length

6,400 bp

Promoter System

T7/SP6 cell-free

Expression System

In-vitro cell-free

Host Strain

Cell-free

Receptor Target

TAS1R2/TAS1R3

IFI Score

713

Pathway Edits

  • Thaumatin II synthetic gene expression cassette with tissue-specific promoter
  • TAS1R2/TAS1R3 orthosteric sweet protein binding site expansion via VFT remodeling
  • TRPM5 conductance amplification via pore-loop S5-P helix engineering
  • Sweet taste receptor allosteric potentiator response element integration
  • Cyclic nucleotide phosphodiesterase 1C suppression cassette for sustained signaling

Key Interventions

Thaumatin disulfide bridge architecture optimization preserving all 8 native disulfidesTAS1R2 CRD-TMD junction interface remodeling for macromolecular ligand accessTRPM5 N-terminal cytoplasmic calmodulin binding domain kinetics modificationReceptor heterodimer interface stabilization via engineered inter-subunit salt bridgesTaste bud progenitor cell differentiation enhancement via Skn-1a regulatory element

IFI Subscale Profile

IFI Profile

IFI-S
713
IFI-U
—
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

next-generation clean-label sweetener development for global food systems

License

$249 · Tier IV License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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