IFI-S · Crystal Grade

Specimen SS-F-SWT-190 // SWEET · IFI-S

Crystal Grade IV-PR-B

Capsicum annuum

Pepper

Tier IVCrystal Grade

Target Trait

8.2×

fold-change

Thaumatin-like sweet protein receptor potency fold-change

Technical Specifications

Genomic Edits

6

GC Content

45.5%

Construct Length

6,560 bp

Promoter System

Ubiquitin / T7

Expression System

Protoplast PEG-fusion

Host Strain

PEG-mediated

Receptor Target

TAS1R2/TAS1R3

IFI Score

820

Pathway Edits

  • Thaumatin II synthetic gene expression cassette with tissue-specific promoter
  • TAS1R2/TAS1R3 orthosteric sweet protein binding site expansion via VFT remodeling
  • TRPM5 conductance amplification via pore-loop S5-P helix engineering
  • Sweet taste receptor allosteric potentiator response element integration
  • Cyclic nucleotide phosphodiesterase 1C suppression cassette for sustained signaling
  • Taste cell-specific transcription factor Skn-1a overexpression for receptor density

Key Interventions

Thaumatin disulfide bridge architecture optimization preserving all 8 native disulfidesTAS1R2 CRD-TMD junction interface remodeling for macromolecular ligand accessTRPM5 N-terminal cytoplasmic calmodulin binding domain kinetics modificationReceptor heterodimer interface stabilization via engineered inter-subunit salt bridgesTaste bud progenitor cell differentiation enhancement via Skn-1a regulatory elementTMEM16A channel gating threshold lowering for amplified depolarization response

IFI Subscale Profile

IFI Profile

IFI-S
820
IFI-U
—
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

Enhanced performance next-generation clean-label sweetener development for global food systems with amplified TAS1R2/TAS1R3 receptor activation for premium-tier applications

License

$299 · Tier IV License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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