Crystal Grade I-BL-B

Capsicum annuum

Pepper

Tier ICrystal Grade

Target Trait

5.17×

fold-change

Thaumatin-like sweet protein receptor potency fold-change

Technical Specifications

Genomic Edits

GC Content

41.9%

Construct Length

4,740 bp

Promoter System

CaMV 35S / Ubiquitin

Expression System

Biolistic transformation

Host Strain

Particle Gun

Receptor Target

TAS1R2/TAS1R3

IFI Score

517

Pathway Edits

Thaumatin II synthetic gene expression cassette with tissue-specific promoter
TAS1R2/TAS1R3 orthosteric sweet protein binding site expansion via VFT remodeling
TRPM5 conductance amplification via pore-loop S5-P helix engineering
Sweet taste receptor allosteric potentiator response element integration

Key Interventions

Thaumatin disulfide bridge architecture optimization preserving all 8 native disulfidesTAS1R2 CRD-TMD junction interface remodeling for macromolecular ligand accessTRPM5 N-terminal cytoplasmic calmodulin binding domain kinetics modificationReceptor heterodimer interface stabilization via engineered inter-subunit salt bridges

IFI Subscale Profile

IFI Profile

IFI-S

517

IFI-U

—

IFI-B

—

IFI-K

—

IFI-C

—

IFI-P

—

IFI-A

—

IFI-F

—

Use Case

Enhanced performance ultra-high-potency natural sweetener platform for industrial food production with amplified TAS1R2/TAS1R3 receptor activation for premium-tier applications

License

$99 · Tier I License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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