IFI-S · Confection Class

Specimen SS-F-SWT-128 // SWEET · IFI-S

Confection Class I-IV-B

Solanum lycopersicum

Tomato

Tier IConfection Class

Target Trait

4.02×

fold-change

Brazzein-analog protein sweetness potency fold-change

Technical Specifications

Genomic Edits

4

GC Content

49.4%

Construct Length

4,740 bp

Promoter System

T7/SP6 cell-free

Expression System

In-vitro cell-free

Host Strain

Cell-free

Receptor Target

TAS1R2/TAS1R3

IFI Score

402

Pathway Edits

  • Brazzein-scaffold sweet protein synthetic gene expression cassette integration
  • TAS1R2 cysteine-rich domain linker region optimization for protein ligand docking
  • Receptor expression density enhancement via codon optimization and Kozak editing
  • Alpha-gustducin translocation efficiency improvement cassette

Key Interventions

Brazzein variant K30R/E53K substitution for enhanced TAS1R2 CRD affinityTAS1R2 N-terminal domain loop remodeling for sweet protein accommodationBeta-arrestin 1 interaction site modification for signal persistenceReceptor internalization suppression via clathrin-mediated endocytosis edit

IFI Subscale Profile

IFI Profile

IFI-S
402
IFI-U
—
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

Enhanced performance protein-based ultra-high-intensity sweetener platform with amplified TAS1R2/TAS1R3 receptor activation for premium-tier applications

License

$99 · Tier I License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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