IFI-S · Nectar Tier

Specimen SS-F-SWT-120 // SWEET · IFI-S

Nectar Tier V-IV-B

Stevia rebaudiana

Stevia

Tier VNectar Tier

Target Trait

6.9×

fold-change

TRPM5 downstream sweet signal amplification fold-change

Technical Specifications

Genomic Edits

6

GC Content

51.7%

Construct Length

7,060 bp

Promoter System

T7/SP6 cell-free

Expression System

In-vitro cell-free

Host Strain

Cell-free

Receptor Target

TAS1R2/TAS1R3

IFI Score

690

Pathway Edits

  • TRPM5 channel expression upregulation in gustatory epithelium cassette
  • TAS1R2/TAS1R3 heterodimer membrane trafficking optimization sequence
  • Phospholipase C beta-2 activity enhancement cassette
  • Phosphodiesterase PDE1A inhibitory response element integration
  • ITPR3 (IP3 receptor type 3) upregulation for enhanced Ca2+ release
  • Gustin (CA6) co-factor overexpression for receptor activity amplification

Key Interventions

TRPM5 gating kinetics optimization via S4-S5 linker modificationReceptor recycling pathway acceleration through endosomal sorting elementInositol phosphate cascade amplification via ITPR3 upregulationTaste bud cell TRPM5 density increase via Skn-1a regulatory elementCa2+ store refilling rate enhancement via SERCA2 co-expression cassetteGustin zinc-binding site optimization for enhanced receptor co-factor activity

IFI Subscale Profile

IFI Profile

IFI-S
690
IFI-U
—
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

Enhanced performance high-performance sweetness modulation for sports nutrition applications with amplified TAS1R2/TAS1R3 receptor activation for premium-tier applications

License

$549 · Tier V License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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