IFI-S · Nectar Tier

Specimen SS-F-SWT-105 // SWEET · IFI-S

Nectar Tier IV-AG-A

Stevia rebaudiana

Stevia

Tier IVNectar Tier

Target Trait

5.13×

fold-change

TRPM5 downstream sweet signal amplification fold-change

Technical Specifications

Genomic Edits

4

GC Content

49.8%

Construct Length

6,240 bp

Promoter System

Tissue-specific 2A11

Expression System

Agrobacterium-mediated T-DNA

Host Strain

EHA105

Receptor Target

TAS1R2/TAS1R3

IFI Score

513

Pathway Edits

  • TRPM5 channel expression upregulation in gustatory epithelium cassette
  • TAS1R2/TAS1R3 heterodimer membrane trafficking optimization sequence
  • Phospholipase C beta-2 activity enhancement cassette
  • Phosphodiesterase PDE1A inhibitory response element integration

Key Interventions

TRPM5 gating kinetics optimization via S4-S5 linker modificationReceptor recycling pathway acceleration through endosomal sorting elementInositol phosphate cascade amplification via ITPR3 upregulationTaste bud cell TRPM5 density increase via Skn-1a regulatory element

IFI Subscale Profile

IFI Profile

IFI-S
513
IFI-U
—
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

taste receptor sensitization platform for reduced sweetener loads

License

$249 · Tier IV License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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