IFI-S · Nectar Tier

Specimen SS-F-SWT-093 // SWEET · IFI-S

Nectar Tier II-PR-A

Stevia rebaudiana

Stevia

Tier IINectar Tier

Target Trait

3.38×

fold-change

TRPM5 downstream sweet signal amplification fold-change

Technical Specifications

Genomic Edits

3

GC Content

47.4%

Construct Length

5,080 bp

Promoter System

Ubiquitin / T7

Expression System

Protoplast PEG-fusion

Host Strain

PEG-mediated

Receptor Target

TAS1R2/TAS1R3

IFI Score

338

Pathway Edits

  • TRPM5 channel expression upregulation in gustatory epithelium cassette
  • TAS1R2/TAS1R3 heterodimer membrane trafficking optimization sequence
  • Phospholipase C beta-2 activity enhancement cassette

Key Interventions

TRPM5 gating kinetics optimization via S4-S5 linker modificationReceptor recycling pathway acceleration through endosomal sorting elementInositol phosphate cascade amplification via ITPR3 upregulation

IFI Subscale Profile

IFI Profile

IFI-S
338
IFI-U
—
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

TRPM5-targeted flavor enhancement in fermented dairy products

License

$99 · Tier II License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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