IFI-S · Nectar Tier

Specimen SS-F-SWT-087 // SWEET · IFI-S

Nectar Tier I-IV-A

Stevia rebaudiana

Stevia

Tier INectar Tier

Target Trait

2.5×

fold-change

TRPM5 downstream sweet signal amplification fold-change

Technical Specifications

Genomic Edits

2

GC Content

46.2%

Construct Length

4,420 bp

Promoter System

T7/SP6 cell-free

Expression System

In-vitro cell-free

Host Strain

Cell-free

Receptor Target

TAS1R2/TAS1R3

IFI Score

250

Pathway Edits

  • TRPM5 channel expression upregulation in gustatory epithelium cassette
  • TAS1R2/TAS1R3 heterodimer membrane trafficking optimization sequence

Key Interventions

TRPM5 gating kinetics optimization via S4-S5 linker modificationReceptor recycling pathway acceleration through endosomal sorting element

IFI Subscale Profile

IFI Profile

IFI-S
250
IFI-U
—
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

plant-derived sweetener potency multiplier for functional beverages

License

$49 · Tier I License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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